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Titolo:
Suppression of maize root diseases caused by Macrophomina phaseolina, Fusarium moniliforme and Fusarium graminearum by plant growth promoting rhizobacteria
Autore:
Pal, KK; Tilak, KVBR; Saxena, AK; Dey, R; Singh, CS;
Indirizzi:
Indian Agr Res Inst, Div Microbiol, New Delhi 110012, India Indian Agr ResInst New Delhi India 110012 biol, New Delhi 110012, India
Titolo Testata:
MICROBIOLOGICAL RESEARCH
fascicolo: 3, volume: 156, anno: 2001,
pagine: 209 - 223
SICI:
0944-5013(2001)156:3<209:SOMRDC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOLOGICAL-CONTROL; PSEUDOMONAS-CEPACIA; BURKHOLDERIA-CEPACIA; RHIZOCTONIA-SOLANI; CYANIDE PRODUCTION; SEED TREATMENT; BACTERIA; RHIZOSPHERE; BIOCONTROL; SIDEROPHORES;
Keywords:
biocontrol; Macrophomina phaseolina; Fusarium graminearum; Fusarium moniliforme; antifungal metabolites;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Pal, KK Natl Res Ctr Groundnut, Ivnagar Rd,BP 5, Junagadh 362001, Gujarat,India Natl Res Ctr Groundnut Ivnagar Rd,BP 5 Junagadh Gujarat India 362001
Citazione:
K.K. Pal et al., "Suppression of maize root diseases caused by Macrophomina phaseolina, Fusarium moniliforme and Fusarium graminearum by plant growth promoting rhizobacteria", MICROBI RES, 156(3), 2001, pp. 209-223

Abstract

A plant growth-promoting isolate of a fluorescent Pseudomonas sp. EM85 andtwo bacilli isolates MR-11(2) and MRF, isolated from maize rhizosphere, were found strongly antagonistic to Fusarium moniliforme, Fusarium graminearum and Macrophomina phaseolina, causal agents of foot rots and wilting, collar rots/stalk rots and root rots and wilting, and charcoal rots of maize, respectively. Pseudomonas sp. EM85 produced antifungal antibiotics (Afa(+)),siderophore (Sid(+)), HCN (HCN+) and fluorescent pigments (Flu(+)) besidesexhibiting plant growth promoting traits like nitrogen fixation, phosphatesolubilization, and production of organic acids and IAA. While MR-11(2) produced siderophore (Sid+), antibiotics (Afa+) and antifungal volatiles (Afv(+)), MRF exhibited the production of antifungal antibiotics (Afa+) and siderophores (Sid+). Bacillus spp. MRF was also found to produce organic acidsand IAA, solubilized tri-calcium phosphate and fixed nitrogen from the atmosphere. All three isolates suppressed the diseases caused by Fusarium moniliforme, Fusarium graminearum and Macrophomina phaseolina in vitro. A Tn5::lacZ induced isogenic mutant of the fluorescent Pseudomonas EM85, M23, along with the two bacilli were evaluated for in situ disease suppression of maize. Results indicated that combined application of the two bacilli significantly (P = 0.05) reduced the Macrophomina-induced charcoal rots of maize by 56.04%. Treatments with the MRF isolate of Bacillus spp. and Tn5:: lacZ mutant (M23) of fluorescent Pseudomonas sp. EM85 significantly reduced collar rots, root and foot rots, and wilting of maize caused by Fusarium moniliforme and F. graminearum (P = 0.05) compared to all other treatments. All these isolates were found very efficient in colonizing the rhizotic zones ofmaize after inoculation. Evaluation of the population dynamics of the fluorescent Pseudomonas sp. EM85 using the Tn5:: lacZ marker and of the Bacillus spp. MRF and MR-11(2) using an antibiotic resistance marker revealed thatall the three isolates could proliferate successfully in the rhizosphere, rhizoplane and endorhizosphere of maize, both at 30 and 60 days after seeding. Four antifungal compounds from fluorescent Pseudomonas sp. EM85, one from Bacillus sp. MR-11(2) and three from Bacillus sp. MRF were isolated, purified and tested in vitro and in thin layer chromatography bioassays. All these compounds inhibited R. solani, M. phaseolina, F. moniliforme, F. graminearum and F. solani strongly. Results indicated that antifungal antibiotics and/or fluorescent pigment of fluorescent Pseudomonas sp. EM85, and antifungal antibiotics of the bacilli along with the successful colonization of all the isolates might be involved in the biological suppression of the maize root diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 20:34:34