Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Pseudomonas fluorescens as a potential pathogen: adherence to nerve cells
Autore:
Picot, L; Abdelmoula, SM; Merieau, A; Leroux, P; Cazin, L; Orange, N; Feuilloley, MGJ;
Indirizzi:
Univ Rouen, UPRES2123, Lab Cold Microbiol, F-27000 Evreux, France Univ Rouen Evreux France F-27000 Cold Microbiol, F-27000 Evreux, France Univ Rouen, UPRES2122, Res Dept Microenvironm & Cell Integrated Renewal, F-76000 Rouen, France Univ Rouen Rouen France F-76000 ntegrated Renewal, F-76000 Rouen, France
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 12, volume: 3, anno: 2001,
pagine: 985 - 995
SICI:
1286-4579(200110)3:12<985:PFAAPP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AERUGINOSA INDUCES APOPTOSIS; MOLECULAR CHARACTERIZATION; EPITHELIAL-CELLS; BINDING-SITES; EXOENZYME-S; PROTEIN; SYSTEM; LIPOPOLYSACCHARIDE; COMPLICATIONS; STRAINS;
Keywords:
Pseudomonas fluorescens; Pseudomonas aeruginosa; neurons; glial cells; adherence; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Feuilloley, MGJ Univ Rouen, UPRES2123, Lab Cold Microbiol, 55,Rue St Germain, F-27000 Evreux, France Univ Rouen 55,Rue St Germain Evreux France F-27000 France
Citazione:
L. Picot et al., "Pseudomonas fluorescens as a potential pathogen: adherence to nerve cells", MICROBES IN, 3(12), 2001, pp. 985-995

Abstract

In order to determine the infectious potential of the psychrotrophic bacterium Pseudomonas fluorescens, a species closely related to the opportunistic pathogen P. aeruginosa, we investigated the binding activity of this bacterium on primary cultures of rat neonate cortical neurons and glial cells, adrenal paraneurons and NG108-15 neuroblastoma cells. Incubated at concentrations of 10(6) and 10(8) CFU/mL, P. fluorescens MF37 exhibited a high binding activity on neurons in the same range as that of P. aeruginosa PAO1. A significant, but lower, adherence of P. fluorescens was also detected on glial cells and adrenal paraneurons. In contrast, when P. fluorescens MF37 orP. aeruginosa PAO1 were incubated,vith neuroblastoma cells, no binding wasobserved. In neurons, the association of P. fluorescens with the plasma membrane occurred both on neurites and cell body. Leakage of the cytoplasmic content was frequently noted. Studies performed using the fluorescent probeHoechst 33258 revealed that in 10% of neurons, P. fluorescens induced the appearance of densely stained clusters of DNA that was typical of an early step of apoptosis. In glial cells exposed to P. fluorescens, marked changesin the morphology of the nucleus, including fragmentation into lobular structures and aggregation of DNA, were also reminiscent of the existence of apossible apoptotic mechanism. Taken together, these results reveal that R fluorescens can bind to nerve cells and affect their physiology and, in agreement with recent clinical observations, suggest that P. fluorescens couldbehave as a pathogen. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:59:04