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Titolo:
Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi 29
Autore:
Morais, MC; Tao, YZ; Olson, NH; Grimes, S; Jardine, PJ; Anderson, DL; Baker, TS; Rossmann, MG;
Indirizzi:
Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Biol Sci, W Lafayette, IN 47907 USA Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Dept Oral Sci, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 al Sci, Minneapolis, MN 55455 USA Univ New Brunswick, Dept Biol, Fredericton, NB E3B 6E1, Canada Univ New Brunswick Fredericton NB Canada E3B 6E1 cton, NB E3B 6E1, Canada
Titolo Testata:
JOURNAL OF STRUCTURAL BIOLOGY
fascicolo: 1, volume: 135, anno: 2001,
pagine: 38 - 46
SICI:
1047-8477(200107)135:1<38:CIROSM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAD-TAIL CONNECTOR; DNA; RNA; PARTICLES; PROTEINS; VIRUS; RELEASE; MODEL;
Keywords:
cryo-EM image reconstruction; phi 29; pRNA; symmetry mismatch; tail appendages;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Rossmann, MG Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Lafayette, IN 47907 USA
Citazione:
M.C. Morais et al., "Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi 29", J STRUCT B, 135(1), 2001, pp. 38-46

Abstract

A method has been developed for three-dimensional image reconstruction of symmetry-mismatched components in tailed phages. Although the method described here addresses the specific case where differing symmetry axes are coincident, the method is more generally applicable, for instance, to the reconstruction of images of viral particles that deviate from icosahedral symmetry. Particles are initially oriented according to their dominant symmetry, thus reducing the search space for determining the orientation of the less dominant, symmetry-mismatched component. This procedure produced an improved reconstruction of the sixfold-symmetric tail assembly that is attached tothe fivefold-symmetric prolate head of phi 29, demonstrating that this method is capable of detecting and reconstructing an object that included a symmetry mismatch. A reconstruction of phi 29 prohead particles using the methods described here establishes that the pRNA molecule has fivefold symmetry when attached to the prohead, consistent with its proposed role as a component of the stator in the phi 29 DNA packaging motor. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:18:40