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Titolo:
NR2B-containing NMDA autoreceptors at synapses on entorhinal cortical neurons
Autore:
Woodhall, G; Evans, DI; Cunningham, MO; Jones, RSG;
Indirizzi:
Univ Bristol, Sch Med Sci, Dept Physiol, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
JOURNAL OF NEUROPHYSIOLOGY
fascicolo: 4, volume: 86, anno: 2001,
pagine: 1644 - 1651
SICI:
0022-3077(200110)86:4<1644:NNAASO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-ASPARTATE RECEPTOR; LONG-TERM POTENTIATION; DEVELOPING NEUROMUSCULAR SYNAPSES; AMINO-ACID RECEPTORS; CORTEX IN-VITRO; SUBUNIT COMPOSITION; GLUTAMATE RELEASE; NEUROTRANSMITTER RELEASE; CEREBRAL-CORTEX; MICE LACKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Woodhall, G Univ Bristol, Sch Med Sci, Dept Physiol, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD 1TD, Avon, England
Citazione:
G. Woodhall et al., "NR2B-containing NMDA autoreceptors at synapses on entorhinal cortical neurons", J NEUROPHYS, 86(4), 2001, pp. 1644-1651

Abstract

We have previously shown that presynaptic N-methyl-D-aspartate receptors (NMDARs) can facilitate glutamate release onto principal neurons in the entorhinal cortex (EC). In the present study, we have investigated the subunit composition of these presynaptic NMDARs. We recorded miniature alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (mEPSCs), from visually identified neurons in layers II and V of the EC in vitro. In both layers, bath application of theNR2A/B subunit-selective agonist, homoquinolinic acid (HQA), resulted in amarked facilitation of mEPSC frequency. Blockade of presynaptic Ca2+ entrythrough either NMDARs or voltage-gated Ca2+ channels with Co2+ prevented the effects of HQA, confirming that Ca2+ entry to the terminal was required for facilitation. When the NR2B-selective antagonist, ifenprodil, was applied prior to HQA, the increase in mEPSC frequency was greatly reduced. In addition, we found that an NMDAR antagonist blocked frequency-dependent facilitation of evoked release and reduced mEPSC frequency in layer V. Thus we have demonstrated that NMDA autoreceptors in layer V of the EC bear the NR2Bsubunit, and that NMDARs are also present at terminals onto superficial neurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:28:41