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Titolo:
Catecholamine release and uptake in the mouse prefrontal cortex
Autore:
Mundorf, ML; Joseph, JD; Austin, CM; Caron, MG; Wightman, RM;
Indirizzi:
Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Chem, Chapel Hill, NC 27599 USA Univ N Carolina, Ctr Neurosci, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 rosci, Chapel Hill, NC 27599 USA Duke Univ, Med Ctr, Howard Hughes Med Inst Labs, Durham, NC USA Duke UnivDurham NC USA Ctr, Howard Hughes Med Inst Labs, Durham, NC USA Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC USA Duke Univ Durham NC USA ke Univ, Med Ctr, Dept Cell Biol, Durham, NC USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 79, anno: 2001,
pagine: 130 - 142
SICI:
0022-3042(200110)79:1<130:CRAUIT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; DOPAMINE UPTAKE; EXTRACELLULAR DOPAMINE; NUCLEUS-ACCUMBENS; FRONTAL-CORTEX; NOREPINEPHRINE TRANSPORTER; NORADRENALINE CARRIER; CYCLIC VOLTAMMETRY; MICE LACKING; RAT STRIATUM;
Keywords:
catecholamines; cyclic voltammetry; immunohistochemical fluorescence; knockout mice; prefrontal cortex; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Wightman, RM Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Hill, NC 27599 USA
Citazione:
M.L. Mundorf et al., "Catecholamine release and uptake in the mouse prefrontal cortex", J NEUROCHEM, 79(1), 2001, pp. 130-142

Abstract

Monitoring the release and uptake of catecholamines from terminals in weakly innervated brain regions is an important step in understanding their importance in normal brain function. To that end, we have labeled brain slicesfrom transgenic mice that synthesize placental alkaline phosphatase (PLAP)on neurons containing tyrosine hydroxylase with antibody-fluorochrome conjugate, PLAP-Cy5. Excitation of the fluorochrome enables catecholamine neurons to be visualized in living tissue. Immunohistochemical fluorescence withantibodies to tyrosine hydroxylase and dopamine beta -hydroxylase revealedthat the PLAP labeling was specific to catecholamine neurons. In the prefrontal cortex (PFC), immunohistochemical fluorescence of the PLAP along withstaining for dopamine transporter (DAT) and norepinephrine transporter (NET) revealed that all three exhibit remarkable spatial overlap. Fluorescencefrom the PLAP antibody was used to position carbon-fiber microelectrodes adjacent to catecholamine neurons in the PFC. Following incubation with L-DOPA, catecholamine release and subsequent uptake was measured and the effectof uptake inhibitors examined. Release and uptake in NET and DAT knockout mice were also monitored. Uptake rates in the cingulate and prelimbic cortex are so slow that catecholamines can exist in the extracellular fluid for sufficient time to travel similar to 100 mum. The results support heterologous uptake of catecholamines and volume transmission in the PFC of mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:43:52