Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Pancreatic beta-cells expressing the Arg64 variant of the beta(3)-adrenergic receptor exhibit abnormal insulin secretory activity
Autore:
Perfetti, R; Hui, H; Chamie, K; Binder, S; Seibert, M; McLenithan, J; Silver, K; Walston, JD;
Indirizzi:
Cedars Sinai Med Ctr, Div Endocrinol, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Los Angeles CA USA 90048 , Los Angeles, CA 90048 USA Cedars Sinai Med Ctr, Dept Pathol, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Los Angeles CA USA 90048 , Los Angeles, CA 90048 USA Johns Hopkins Univ, Dept Geriatr Med & Gerontol, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA tr Med & Gerontol, Baltimore, MD USA Univ Maryland, Div Endocrinol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Endocrinol, Baltimore, MD 21201 USA
Titolo Testata:
JOURNAL OF MOLECULAR ENDOCRINOLOGY
fascicolo: 2, volume: 27, anno: 2001,
pagine: 133 - 144
SICI:
0952-5041(200110)27:2<133:PBETAV>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT DIABETES-MELLITUS; BETA-3-ADRENERGIC RECEPTOR; TRP64ARG MUTATION; GENETIC-VARIATION; ADRENOCEPTOR AGONIST; TISSUE DISTRIBUTION; OBESITY; HUMANS; ADIPOCYTES; RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Perfetti, R Cedars Sinai Med Ctr, Div Endocrinol & Metab, 8723 Alden Dr,SSB 290, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr 8723 Alden Dr,SSB 290Los Angeles CA USA 90048
Citazione:
R. Perfetti et al., "Pancreatic beta-cells expressing the Arg64 variant of the beta(3)-adrenergic receptor exhibit abnormal insulin secretory activity", J MOL ENDOC, 27(2), 2001, pp. 133-144

Abstract

The Arg64 beta (3)-adrenergic receptor (beta (3)AR) variant is associated with an earlier age of onset of diabetes and lower levels of insulin secretion in humans. The aims of this study were to investigate whether beta (3)AR is expressed by islet cells, if receptor binding affects insulin secretion and, finally, if the beta (3)AR Arg64 variant induces abnormal insulin secretory activity. Human pancreas extracts were subjected to RT-PCR, Westernblotting and immunostaining analyses. DNA sequencing and Western blotting demonstrated that the beta (3)AR gene is transcribed and translated in the human pancreas; immunostaining showed that it is expressed by the islets ofLangerhans. Cultured rat beta -cells responded to human beta (3)AR agonists in a dose- and time-dependent manner. Transfection of cultured rat beta -cells with the wild-type human beta (3)AR produced an increased baseline and ligand-dependent insulin secretion compared with parental cells. On the other hand, cells transfected with the Arg64 variant of the beta (3)AR secreted less insulin, both spontaneously and after exposure to human beta (3)ARagonists. Furthermore, while transfection with the wild-type beta (3)AR preserved the glucose-dependent secretion of insulin, expression of the variant receptor rendered the host cells significantly less responsive to glucose. In summary, cells express the beta (3)AR, and its activation contributesto the regulation of insulin secretion. These findings may help explain the low levels of insulin secretion in response to an glucose tolerance test observed in humans carrying the Arg64 polymorphism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:29:33