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Titolo:
Intermolecular H-1{F-19} NOEs in studies of fluoroalcohol-induced conformations of peptides and proteins
Autore:
Martinez, D; Gerig, JT;
Indirizzi:
Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USAUniv Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA
Titolo Testata:
JOURNAL OF MAGNETIC RESONANCE
fascicolo: 2, volume: 152, anno: 2001,
pagine: 269 - 275
SICI:
1090-7807(200110)152:2<269:IHNISO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
EGG-WHITE LYSOZYME; SECONDARY STRUCTURE; SELF-ASSOCIATION; TRIFLUOROETHANOL; ALCOHOLS; WATER; SPECTROSCOPY; ASSIGNMENTS; RESONANCE; DYNAMICS;
Keywords:
intermolecular NOE; lysozyme; trifluoroethanol; fluoroalcohol; TFE state;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Gerig, JT Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 A 93106 USA
Citazione:
D. Martinez e J.T. Gerig, "Intermolecular H-1{F-19} NOEs in studies of fluoroalcohol-induced conformations of peptides and proteins", J MAGN RES, 152(2), 2001, pp. 269-275

Abstract

Mixtures of fluorinated alcohols and water can selectively stabilize certain secondary structures of peptides and proteins. Such mixtures may also beof use in solubilizing hydrophobic or membrane-bound proteins. We show that intermolecular dipolar interactions between the fluorine nuclei of such solvents and the protons of a dissolved protein lead to readily detected H-1{F-19} nuclear Overhauser effects. These NOEs can potentially provide information about solvent exposure of particular groups as well as indicate theformation of long-lived fluoroalcohol-solute complexes. Results obtained with HEW lysozyme in solutions containing trifluoroethanol illustrate these possibilities. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:25:51