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Titolo:
Double quantum filtered H-1 NMR spectroscopy enables quantification of lactate in muscle
Autore:
Asllani, I; Shankland, E; Pratum, T; Kushmerick, M;
Indirizzi:
Univ Washington, Dept Bioengn, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Bioengn, Seattle, WA 98195 USA Univ Washington, Dept Radiol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 , Dept Radiol, Seattle, WA 98195 USA Univ Washington, Dept Chem, Seattle, WA 98195 USA Univ Washington SeattleWA USA 98195 on, Dept Chem, Seattle, WA 98195 USA Univ Washington, Dept Physiol & Biophys, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 iol & Biophys, Seattle, WA 98195 USA
Titolo Testata:
JOURNAL OF MAGNETIC RESONANCE
fascicolo: 2, volume: 152, anno: 2001,
pagine: 195 - 202
SICI:
1090-7807(200110)152:2<195:DQFHNS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCISED RAT MUSCLE; DIPOLAR;
Keywords:
double quantum; NMR spectroscopy; lactate; dipolar coupling; scalar coupling; transverse relaxation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Asllani, I Univ Washington, Dept Bioengn, Mail Box 357115,1959 NE Pacific Ave, Seattle, WA 98195 USA Univ Washington Mail Box 357115,1959 NE Pacific Ave Seattle WA USA 98195
Citazione:
I. Asllani et al., "Double quantum filtered H-1 NMR spectroscopy enables quantification of lactate in muscle", J MAGN RES, 152(2), 2001, pp. 195-202

Abstract

In this study we address the question of quantification of muscle lactate using double quantum filtered (DQF) H-1 NMR spectroscopy where dipolar and scalar coupled spectra are acquired. For this, lactate content in muscle samples was independently determined using a conventional enzymatic assay andDQF, H-1 NMR spectroscopy. NMR quantification of lactate relied on comparison of muscle spectra with similarly acquired spectra of standard lactate solutions. Transverse relaxation, T-2, and dipolar coupling effects were investigated at two different orientations of muscle fibers relative to B-o and at various lactate concentrations. In all cases, we found a biexponentialT-2 decay of the lactate methyl signal with a long T-2 of 142 ms (+/-8 ms,n=24) and a short T-2 of 37 ms (+/-6 ms, n=24). Lactate content of muscle determined by NMR spectroscopy agreed with the results obtained from enzymatic assays of the same samples provided that T-2 effects as well as the presence of both scalar and dipolar coupling interactions of lactate in musclewere taken into account. (C) 2001 Academic Press.

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Documento generato il 01/04/20 alle ore 01:51:26