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Titolo:
Morphine modulates lymph node-derived T lymphocyte function: role of caspase-3,-8, and nitric oxide
Autore:
Wang, JH; Charboneau, R; Balasubramanian, S; Barke, RA; Loh, HH; Roy, S;
Indirizzi:
Vet Affairs Med Ctr, Dept Surg, Minneapolis, MN 55417 USA Vet Affairs Med Ctr Minneapolis MN USA 55417 g, Minneapolis, MN 55417 USA Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA N Mem Med Ctr, Robbinsdale, MN USA N Mem Med Ctr Robbinsdale MN USAN Mem Med Ctr, Robbinsdale, MN USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 4, volume: 70, anno: 2001,
pagine: 527 - 536
SICI:
0741-5400(200110)70:4<527:MMLNTL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MU-OPIOID RECEPTOR; MACROPHAGE APOPTOSIS; PERIPHERAL-BLOOD; OPIATE RECEPTORS; KNOCKOUT MICE; IN-VIVO; CELLS; ACTIVATION; PROLIFERATION; SUPPRESSION;
Keywords:
mu-opioid receptor knock-out; wild type; apoptosis; induced nitric oxide synthase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, JH Vet Affairs Med Ctr, Dept Surg, Res RT 151,Room 3N 107,1 Vet Dr, Minneapolis, MN 55417 USA Vet Affairs Med Ctr Res RT 151,Room 3N 107,1 Vet Dr Minneapolis MN USA 55417
Citazione:
J.H. Wang et al., "Morphine modulates lymph node-derived T lymphocyte function: role of caspase-3,-8, and nitric oxide", J LEUK BIOL, 70(4), 2001, pp. 527-536

Abstract

The major objective of this paper is to characterize the mechanism by which morphine modulates lymphocyte function and if these effects are mediated through the Ut-opioid receptor. We evaluated the in vitro effects of morphine on lymphocytes. that were freshly isolated from lymph nodes from wild type (WT) and Vc-opioid receptor knock-out (MORKO) mice. Results show that morphine inhibits Con A-induced lymph node T-cell proliferation and IL-2 and IFN-gamma synthesis in a dose-dependent manner. This effect was abolished in lymph node cells isolated from MORKO mice. The inhibition of T-cell function with low-dose morphine was associated with an increase in caspase-3- and caspase-8-mediated apoptosis. The inhibition of T-cell function with high-dose morphine was associated with an increase in the inducible NO synthasemRNA expression. N-G-nitro-L-arginine methyl ester (L-NAME) antagonized the apoptosis. induced by high-dose morphine. Our results suggest that low-dose morphine, through the IL-opioid receptor, can induce lymph node lymphocyte apoptosis through the cleavage activity of caspase-3 and caspase-8. Morphine at high doses induces NO release. This effect of morphine is also mediated through the ft-opioid receptor present on the surface of macrophages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:21:04