Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The A-factor regulatory cascade and cAMP in the regulation of physiological and morphological development in Streptomyces griseus
Autore:
Horinouchi, S; Ohnishi, Y; Kang, DK;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 otechnol, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
fascicolo: 3, volume: 27, anno: 2001,
pagine: 177 - 182
SICI:
1367-5435(200109)27:3<177:TARCAC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
AERIAL MYCELIUM FORMATION; SECONDARY METABOLISM; FACTOR RECEPTOR; COELICOLOR A3(2); CELLULAR-DIFFERENTIATION; ANTIBIOTIC PRODUCTION; FACTOR BIOSYNTHESIS; STRINGENT RESPONSE; PROTEIN-KINASE; GENE;
Keywords:
A-factor receptor protein; signal transduction; streptomycin biosynthesis; aerial hyphae formation; stringent response factor ppGpp; protein tyrosine phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Horinouchi, S Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, BunkyoKu, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 yo Ku, Tokyo 1138657, Japan
Citazione:
S. Horinouchi et al., "The A-factor regulatory cascade and cAMP in the regulation of physiological and morphological development in Streptomyces griseus", J IND MIC B, 27(3), 2001, pp. 177-182

Abstract

In the A-factor regulatory cascade leading to the onset of streptomycin biosynthesis and aerial mycelium formation in Streptomyces griseus, the A-factor receptor protein (ArpA) serves as a DNA-binding repressor and A-factor releases the repression by binding to ArpA and dissociating it from the DNA. Mutants defective in arpA therefore produce streptomycin and aerial hyphae in the absence of A-factor. A gene that inhibits streptomycin production and aerial hyphae formation in an arpA mutant was cloned on a high-copy-number plasmid and found to encode a eukaryotic-type adenylate cyclase (CyaA). Consistent with this, an exogenous supply of cAMP at high concentration almost abolished streptomycin production and aerial hyphae formation. On the other hand, cAMP at lower concentrations stimulated or accelerated these developmental processes. The effects of cAMP were detectable only in arpA mutants, nd not in the wild-type strain; an exogenous supply of cAMP or cyaA disruption in the wild-type strain caused almost no effect on these phenotypes. Thus the effects of cAMP became apparent only in the arpA-defective background. cAMP at high concentrations inhibited stringent response factor ppGpp production, which is important for the onset of antibiotic biosynthesis. cAMP also influenced the timing of tyrosine phosphorylation of more than nine proteins. These findings show that a cAMP regulatory relay for physiological and morphological development functions in a concerted and interdependent way with other signal transduction pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:24:31