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Titolo:
Development of a novel method for operating magnetic particles, magtrationtechnology, and its use for automating nucleic acid purification
Autore:
Obata, K; Segawa, O; Yakabe, M; Ishida, Y; Kuroita, T; Ikeda, K; Kawakami, B; Kawamura, Y; Yohda, M; Matsunaga, T; Tajima, H;
Indirizzi:
Toyobo Co Ltd, Tsuruga Inst Biotechnol, Fukui 9140047, Japan Toyobo Co Ltd Fukui Japan 9140047 Inst Biotechnol, Fukui 9140047, Japan Precis Syst Sci Co Ltd, Matsudo, Chiba 2710064, Japan Precis Syst Sci Co Ltd Matsudo Chiba Japan 2710064 , Chiba 2710064, Japan Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan Tokyo Univ Agr & Technol Koganei Tokyo Japan 1848588 Tokyo 1848588, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 5, volume: 91, anno: 2001,
pagine: 500 - 503
SICI:
1389-1723(200105)91:5<500:DOANMF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID DNA; ALKALINE EXTRACTION;
Keywords:
magnetic separation; beads; nucleic acid; automation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Tajima, H Toyobo Co Ltd, Tsuruga Inst Biotechnol, 10-24 Toyo Cho, Fukui 9140047, Japan Toyobo Co Ltd 10-24 Toyo Cho Fukui Japan 9140047 9140047, Japan
Citazione:
K. Obata et al., "Development of a novel method for operating magnetic particles, magtrationtechnology, and its use for automating nucleic acid purification", J BIOSCI BI, 91(5), 2001, pp. 500-503

Abstract

Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recoveryof magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 06:53:56