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Titolo:
Structural analysis of the sugar chains of human urinary thrombomodulin
Autore:
Wakabayashi, H; Natsuka, S; Honda, M; Naotsuka, M; Ito, Y; Kajihara, J; Hase, S;
Indirizzi:
Osaka Univ, Dept Chem, Grad Sch Sci, Toyonaka, Osaka 5600043, Japan Osaka Univ Toyonaka Osaka Japan 5600043 i, Toyonaka, Osaka 5600043, Japan Mochida Pharmaceut Co Ltd, Res Ctr, Shizuoka 4128524, Japan Mochida Pharmaceut Co Ltd Shizuoka Japan 4128524 Shizuoka 4128524, Japan Mochida Pharmaceut Co Ltd, Pharmaceut Lab, Fujieda, Shizuoka 4268640, Japan Mochida Pharmaceut Co Ltd Fujieda Shizuoka Japan 4268640 a 4268640, Japan JCR Pharmaceut Co Ltd, Res Ctr, Kobe, Hyogo 6512241, Japan JCR Pharmaceut Co Ltd Kobe Hyogo Japan 6512241 Kobe, Hyogo 6512241, Japan
Titolo Testata:
JOURNAL OF BIOCHEMISTRY
fascicolo: 4, volume: 130, anno: 2001,
pagine: 543 - 552
SICI:
0021-924X(200110)130:4<543:SAOTSC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT HUMAN THROMBOMODULIN; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLUBLE THROMBOMODULIN; HUMAN-PLASMA; FACTOR-VII; FACTOR-IX; IDENTIFICATION; GLYCOPROTEINS; CARBOHYDRATE; COAGULATION;
Keywords:
N-linked sugar chain; O-linked sugar chain; thrombomodulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hase, S Osaka Univ, Dept Chem, Grad Sch Sci, Toyonaka, Osaka 5600043, Japan Osaka Univ Toyonaka Osaka Japan 5600043 aka, Osaka 5600043, Japan
Citazione:
H. Wakabayashi et al., "Structural analysis of the sugar chains of human urinary thrombomodulin", J BIOCHEM, 130(4), 2001, pp. 543-552

Abstract

The sugar chains of human urinary thrombomodulin were studied. N- and O-linked sugar chains were simultaneously liberated by hydrazinolysis followed by N-acetylation and were tagged with 2-aminopyridine. Then the structures of the N- and O-linked pyridylamino (PA-) sugar chains were analyzed by two-dimensional sugar mapping combined with exoglycosidase digestion. The major N-linked sugar chains of human urinary thrombomodulin were found to be monosialo- and disialofucosylbiantennary chains, while the major O-linked sugar chain was +/- Sia alpha2-3Gal beta1-3(+/- Sia alpha2-6)GalNAc. Thrombomodulin also contained the reported structure SO4-3GlcA beta1-3Gal beta1-3(+/- Sia alpha2-6)Gal beta1-4Xyl [H. Wakabayashi, S. Natsuka, T. Mega, N. Otsuki, AL Isaji, M. Naotsuka, S. Koyama, T. Kanamori, M Sakai, and S. Hase (1999) J. Biol. Chem. 274, 5436-5442]. In addition to these sugar chains, a single Glc was linked to Ser 287.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 18:23:10