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Titolo:
Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET
Autore:
Lee, KS; Joo, BS; Na, YJ; Yoon, MS; Choi, OH; Kim, WW;
Indirizzi:
Pusan Natl Univ, Sch Med, Dept Obstet & Gynecol, Seoku, Pusan 602739, South Korea Pusan Natl Univ Pusan South Korea 602739 eoku, Pusan 602739, South Korea
Titolo Testata:
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
fascicolo: 9, volume: 18, anno: 2001,
pagine: 490 - 498
SICI:
1058-0468(200109)18:9<490:CCAAAI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; HUMAN EMBRYO DEVELOPMENT; CHROMOSOMAL-ABNORMALITIES; INVITRO FERTILIZATION; MULTIPLE PREGNANCIES; MEMBRANA GRANULOSA; AGED MICE; IMPLANTATION; COCULTURE; FAILURE;
Keywords:
apoptosis; cumulus cells; oocyte quality; outcome of IVF-ET;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, KS Pusan Natl Univ, Sch Med, Dept Obstet & Gynecol, Seoku, 1-10 Amidong, Pusan 602739, South Korea Pusan Natl Univ 1-10 Amidong Pusan South Korea 602739 South Korea
Citazione:
K.S. Lee et al., "Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET", J AS REPROD, 18(9), 2001, pp. 490-498

Abstract

Purpose. Our purpose was to establish an evaluation system for oocyte quality based on the incidence of cumulus cells apoptosis and to examine the effect of coculture, using autologous cumulus cells, on the outcome of IVF-ETaccording to proliferative activities of helper cells and the incidence ofcumulus cells apoptosis. Methods. Cumulus cell masses were collected from 91 mature oocytes among 330 oocytes retrieved fi-om a total of 34 IVF-ET cycles with tubal infertility and unexplained infertility. The incidence of apoptosis in cumulus cellswas assessed by apoptosis detection kit fluorescein. On ovum pick tip, 2ndday embryos were cocultured with autologous cumulus cells. Prior to coculture, in vitro proliferative activity of cumulus cells was evaluated. Results. Cumulus cells from patient groups over 40 years old had a significantly increased apoptosis incidence, a lower fertilization rate, and the decreased number of oocytes retrieved compared to the other age groups (P < .05). The incidence of cumulus cells apoptosis was significantly lower whenthe number of oocytes retrieved was 5 or less (P < .05). Cumulus cells from fertilized oocytes (0.43 +/- 0.07%) and those from patients who became pregnant (0.44 +/- 0.11%) following IVF-ET showed a significantly lower incidence of apoptosis compared to those of unfertilized oocytes (1.80 +/- 0.35%;, P < .001) and the nonpregnant group (0.81 +/- 0.10%; P < .05). Embryo quality also had a negative correlation with the incidence of cumulus cells apoptosis. Coculture of fertilized oocytes with cumulus cells with high proliferative activity resulted in improved rates of implantation and pregnancycompared to that with poor active cumulus cells. No significant differencewas found between the in vitro proliferative activity of cumulus cells andthe incidence of cumulus cells apoptosis (P < .063). Conclusions: The age of women might influence the incidence of apoptosis in cumulus cells, and the increased incidence of apoptosis is associated with the number of oocytes retrieved, the fertilization rate, and the pregnancy outcome following IVF-ET These results suggest that the incidence of cumulus cells apoptosis can be used in predicting oocyte quality., outcome of IVF-ET, and age-related decline in fertility.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 14:10:24