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Titolo:
Direct shoot organogenesis and plant regeneration in safflower
Autore:
Mandal, AKA; Gupta, SD;
Indirizzi:
Natl Bot Res Inst, Floriculture Sect, Lucknow 226001, Uttar Pradesh, IndiaNatl Bot Res Inst Lucknow Uttar Pradesh India 226001 Uttar Pradesh, India Indian Inst Technol, Dept Agr & Food Engn, Kharagpur 721302, W Bengal, India Indian Inst Technol Kharagpur W Bengal India 721302 1302, W Bengal, India
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 1, volume: 37, anno: 2001,
pagine: 50 - 54
SICI:
1054-5476(200101/02)37:1<50:DSOAPR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARTHAMUS-TINCTORIUS L; CULTURE;
Keywords:
Carthamus tinctorius L.; histology; in vitro shoot; adventitious origin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Mandal, AKA Natl Bot Res Inst, Floriculture Sect, Lucknow 226001, Uttar Pradesh, India Natl Bot Res Inst Lucknow Uttar Pradesh India 226001 h, India
Citazione:
A.K.A. Mandal e S.D. Gupta, "Direct shoot organogenesis and plant regeneration in safflower", IN VITRO-PL, 37(1), 2001, pp. 50-54

Abstract

Adventitious shoot buds were induced directly on the adaxial surface of the cotyledons of eight safflower cultivars after 14 d of culture initiation on Murashige and Skoog's (MS) medium supplemented with various levels of 6-benzylaminopurine (BA). Maximum shoot organogenesis of 54.4% with 10.2 shoots per responding cotyledon was obtained with 8.87 muM BA in the cv. S-144. Regenerated shoots were classified into three groups on the basis of theirmorphological features and were found to be correlated with the levels of BA. The highest number of normal shoots was obtained from 2.2 muM BA treatment. The regenerated shoots of group I (normal shoots) were rooted on half-strength MS medium supplemented with 5.3 muM alpha -naphthaleneacetic acid,3% sucrose and 0.8% bacto-agar. Rooted plantlets were successfully transferred to soil and appeared morphologically normal. Histological studies revealed that shoot buds originated adventitiously from subepidermal cells.

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Documento generato il 11/08/20 alle ore 15:35:08