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Titolo:
Manipulation of the morphogenetic pathways of Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin
Autore:
Nhut, DT; Le, BV; Van, KTT;
Indirizzi:
Inst Biol Dalat, Da Lat, Lam Dong, Vietnam Inst Biol Dalat Da Lat Lam Dong Vietnam Dalat, Da Lat, Lam Dong, Vietnam Vietnam Natl Univ, Coll Nat Sci, Fac Biol, Dept Biotechnol, Thanh Pho Ho Chi Minh, Vietnam Vietnam Natl Univ Thanh Pho Ho Chi Minh Vietnam ho Ho Chi Minh, Vietnam Univ Paris 11, UMR 8618, Inst Biotechnol Plantes, F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 echnol Plantes, F-91405 Orsay, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 1, volume: 37, anno: 2001,
pagine: 44 - 49
SICI:
1054-5476(200101/02)37:1<44:MOTMPO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; CULTURE; INVITRO; THUNB;
Keywords:
bulblet; callus; Lilium longiflorum; explant position; protocorm-like body; regeneration; root; shoot; thin cell layer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Nhut, DT Hiragi 407-6, Miki, Kagawa, Japan Hiragi 407-6 Miki Kagawa Japan iragi 407-6, Miki, Kagawa, Japan
Citazione:
D.T. Nhut et al., "Manipulation of the morphogenetic pathways of Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin", IN VITRO-PL, 37(1), 2001, pp. 44-49

Abstract

Excised tissues from transverse young stem sections of Lilium longiflorum were cultured on Murashige and Skoog medium supplemented with growth regulators at various concentrations. After 45 d in culture, the presence of alpha -naphthaleneacetic acid (NAA) in the culture medium at 5.4 muM resulted in bulblet formation while 2,4-dichlorophenoxyacetic acid (2,4-D) at 2.2 muMresulted in root formation. The presence of IBA (indole-3-butyric acid) inthe culture medium at 1.0 muM resulted in shoot formation while plantlet formation occurred when IBA was added at a concentration of 2.0 muM. When 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) was added to the culture mediumat 1.1 muM, protocorm-like bodies (PLBs) formed, while 2.2 LM resulted in shoot formation (on abaxial and adaxial surfaces). The presence of NAA and TDZ in the culture medium at 5.4 muM and 0.4, 1.1 or 2.2 muM, respectively,resulted in somatic embryo formation while, NAA- and 6-benzylaminopurine- (BA) containing culture medium formed callus or bulblets. The establishmentof different regeneration systems when explants are exposed to various growth regulators demonstrates that the choice of growth regulator combinations and concentrations are of significance in determining the morphogenetic response and plant regeneration capacity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 23:08:47