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Titolo:
Quercus suber L. somatic embryo germination and plant conversion: Pretreatments and germination conditions
Autore:
Garcia-Martin, G; Gonzalez-Benito, ME; Manzanera, JA;
Indirizzi:
Escuela Univ Ingn Tecn Agr, Dept Biol Vegetal, Madrid 28040, Spain EscuelaUniv Ingn Tecn Agr Madrid Spain 28040 getal, Madrid 28040, Spain Inst Madrileno Invest Agr, Madrid 28800, Spain Inst Madrileno Invest Agr Madrid Spain 28800 st Agr, Madrid 28800, Spain Univ Politecn Madrid, Dept Silvopascicultura, E-28040 Madrid, Spain Univ Politecn Madrid Madrid Spain E-28040 cultura, E-28040 Madrid, Spain
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 2, volume: 37, anno: 2001,
pagine: 190 - 198
SICI:
1054-5476(200103/04)37:2<190:QSLSEG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROBUR L; INTERIOR SPRUCE; ABSCISIC-ACID; EMBRYOGENESIS; REGENERATION; SEEDS; MATURATION; INDUCTION; CULTURE;
Keywords:
chilling; cork oak; plantlet; priming; in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Gonzalez-Benito, ME Escuela Univ Ingn Tecn Agr, Dept Biol Vegetal, Ciudad Univ, Madrid 28040, Spain Escuela Univ Ingn Tecn Agr Ciudad Univ Madrid Spain 28040
Citazione:
G. Garcia-Martin et al., "Quercus suber L. somatic embryo germination and plant conversion: Pretreatments and germination conditions", IN VITRO-PL, 37(2), 2001, pp. 190-198

Abstract

Osmoticum (high sucrose concentration) and chilling (4 degreesC) treatments were assayed in order to promote germination (radicle elongation) and plant conversion of Quercus suber somatic embryos. Those treatments were applied before embryos were set to germinate on Murashige and Skoog (MS) liquid medium. Chilling for 2 mo. improved germination rate (best results close to100%). On the other hand, that treatment increased plant conversion, especially when high sucrose concentration was used. The best results were obtained with 150 g l(-1) sucrose (75% of cultured embryos converted to plants). Incubation conditions during germination were also studied. Although therewere no significant differences on final results, warmer temperatures and darkness increased germination rate. The influence of the different treatments on shoot morphology was also studied. The highest percentages of shootswith normal leaves obtained were close to 25%.

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Documento generato il 19/09/20 alle ore 18:38:28