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Titolo:
Strategies for expressing multiple foreign genes in plants as polycistronic constructs
Autore:
Hunt, AG; Maiti, IB;
Indirizzi:
Univ Kentucky, Dept Agron, Lexington, KY 40546 USA Univ Kentucky Lexington KY USA 40546 Dept Agron, Lexington, KY 40546 USA Univ Kentucky, Tobacco & Hlth Res Inst, Lexington, KY 40546 USA Univ Kentucky Lexington KY USA 40546 th Res Inst, Lexington, KY 40546 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 3, volume: 37, anno: 2001,
pagine: 313 - 320
SICI:
1054-5476(200105/06)37:3<313:SFEMFG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAULIFLOWER MOSAIC-VIRUS; TRANSGENIC TOBACCO PLANTS; THALIANA ROOT EXPLANTS; RIBOSOME ENTRY SITE; BIOSYNTHETIC-PATHWAY; CARBONIC-ANHYDRASE; PRECURSOR PROTEIN; TRANSLATION; VI; CHLOROPLASTS;
Keywords:
multigene expression; polycistronic mRNA; plant biotechnology;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Hunt, AG Univ Kentucky, Dept Agron, N212A Agr Sci Ctr N,500 S Limestone St, Lexington, KY 40546 USA Univ Kentucky N212A Agr Sci Ctr N,500 S LimestoneSt Lexington KY USA 40546
Citazione:
A.G. Hunt e I.B. Maiti, "Strategies for expressing multiple foreign genes in plants as polycistronic constructs", IN VITRO-PL, 37(3), 2001, pp. 313-320

Abstract

With an ever-increasing frequency, it has become desirable to express manyforeign genes in the same transgenic plant. A range of approaches to this problem have been explored in the period since gene transfer was developed as a useful tool in plant science. These have ranged from assembly of multiple transcription units on a single DNA vector, to the execution of severalindependent transformations involving single genes followed by successive rounds of hybridization to yield plant lines with the desired combination of genes, to cotransformation with multiple plasmids followed by molecular screening to identify desired transgenic plants. Recent developments have provided new alternatives to the problem of the expression of multiple foreign genes in plants, approaches that revolve around the expression of polycistronic mRNAs. This review summarizes the current status of the area of polycistronic gene expression in transgenic plants, within the context of multigene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:22:25