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Titolo:
Production of male sterile interspecific somatic hybrids between transgenic N-tabacum (bar) and N-rotundifolia (npt II) and their identification by AFLP analysis
Autore:
Ilcheva, V; San, LH; Zagorska, N; Dimitrov, B;
Indirizzi:
Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 Genet, BU-1113 Sofia, Bulgaria SEITA, Tobacco Inst, F-24100 Bergerac, France SEITA Bergerac France F-24100 TA, Tobacco Inst, F-24100 Bergerac, France
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 4, volume: 37, anno: 2001,
pagine: 496 - 502
SICI:
1054-5476(200107/08)37:4<496:POMSIS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NICOTIANA-TABACUM; PROTOPLAST FUSION; TRANSLOCATION EVENTS; INSITU HYBRIDIZATION; GAMMA-FUSION; RESISTANCE; PLANTS; PLUMBAGINIFOLIA; DEBNEYI; POTATO;
Keywords:
protoplast fusion; tobacco; ploidy; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ilcheva, V Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 113 Sofia, Bulgaria
Citazione:
V. Ilcheva et al., "Production of male sterile interspecific somatic hybrids between transgenic N-tabacum (bar) and N-rotundifolia (npt II) and their identification by AFLP analysis", IN VITRO-PL, 37(4), 2001, pp. 496-502

Abstract

A protoplast fusion experiment was carried out aiming to obtain somatic hybrid plants of transgenic Nicotiana tabacum (bar) (+) N. rotundifolia (npt II). The bialaphos resistance marker (bar) was introduced into A tabacum via Agrobacterium tumefaciens using vector pGV1500 carrying the bar gene phosphinothricin acetyltransferase. A rotundifolia (npt II) was recovered afterdirect gene transformation of protoplasts by the pGP6 plasmid carrying thenpt II gene for neomycin phosphotransferase. Both plasmids possessed 35S CaMV promoters. Hybrid selection was based on dual bialaphoskanamycin resistance. Amplified fragment length polymorphism (AFLP) analysis of regeneratedplants showed the presence of species-specific bands for both parents, which confirmed their hybrid nature. IV. tabacum (bar) (+) N. rotundifolia (npt II) hybrids exhibited a great diversity in morphology. Fertile hybrids which possessed N. tabacum or N. rotundifolia morphology were recovered. Flowcytometric analysis revealed that the N. tabacum- and N. rotundifolia-likehybrids had nuclear DNA contents near that of N. tabacum (9.40 +/- 0.24 pg) or N. rotundifolia (5.29 +/- 0.36 pg), respectively, and were highly asymmetric. Other hybrids combined traits from the two species at various levels - N. tabacum habit or branched, similar to IV rotundifolia. Their leaves varied in shape. The flowers of the hybrid plants were of N. tabacum or IV.rotundifolia type, or had N. rotundifolia dimensions, pink with N. tabacumcorolla or white with curly fused petals. All were self-sterile or male sterile. The nuclear DNA content varied from 8.90 +/- 0.30 to 19.57 +/- 0.33 pg. The data from the morphological and cytological analysis provided evidence that parental chromosome elimination in the hybrid clones was spontaneous and not species-specific and that diploidization of the tobacco genome might have occurred in some clones during in vitro culture. This reflects the genomic incompatibility between the two species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:08:33