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Titolo:
Bromodeoxyuridine increases keratin 19 protein expression at a posttranscriptional level in two human lung tumor cell lines
Autore:
Meleady, P; Clynes, M;
Indirizzi:
Dublin City Univ, Natl Cell & Tissue Culture Ctr, Dublin 9, Ireland DublinCity Univ Dublin Ireland 9 Tissue Culture Ctr, Dublin 9, Ireland
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 8, volume: 37, anno: 2001,
pagine: 536 - 542
SICI:
1071-2690(200109)37:8<536:BIK1PE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; AMINO-ACID-SEQUENCE; CYTOKERATIN GENE-EXPRESSION; INTERMEDIATE FILAMENTS; CARCINOMA-CELLS; DOWN-REGULATION; CULTURED HUMAN; RETINOIC ACID; CANCER-CELLS; STEM-CELLS;
Keywords:
bromodeoxyuridine; keratin 19; lung; differentiation; posttranscriptional control;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Meleady, P Dublin City Univ, Natl Cell & Tissue Culture Ctr, Dublin 9, Ireland Dublin City Univ Dublin Ireland 9 ture Ctr, Dublin 9, Ireland
Citazione:
P. Meleady e M. Clynes, "Bromodeoxyuridine increases keratin 19 protein expression at a posttranscriptional level in two human lung tumor cell lines", IN VITRO-AN, 37(8), 2001, pp. 536-542

Abstract

Keratins form the largest subfamily of intermediate filament proteins and show strict lineage- and differentiation-associated expression in epithelial cells. Little is known about the mechanisms that control keratin protein synthesis in these cells. We have examined the effect of the differentiation-modulating agent, 5'-bromo-2'-deoxyuridine (BrdU), on keratin 19 (K19) expression in two human lung carcinoma cell lines, DLKP and A549. Treatment of both cell lines with 10 muM BrdU for 7 d induced the expression of K19 protein in keratin-negative DLKP cells, and significantly increased K19 protein expression in A549 cells. K19 messenger ribonucleic acids (mRNAs) were detected by Northern blot and reverse transcriptase-polymerase chain reaction analyses in both cell lines, but no increase in K19 mRNA levels was detected in either cell line, even with treatment with BrdU for up to 21 d. Thissuggests that K19 protein synthesis is normally blocked at a posttranscriptional level in DLKP cells, and BrdU can somehow reverse this block, resulting in the induction of K19 protein synthesis. Treatment of HL60, a leukemic cell line, with BrdU, resulted in noninduction of K19 protein synthesis, and no K19 mRNA transcripts were detected before and after BrdU treatment, possibly suggesting that BrdU is acting in an epithelial-specific manner toreverse a block in K19 protein synthesis in DLKP keratin-negative lung cancer cells. Therefore, DLKP (and A549) may be useful Cellular models to investigate if this represents a regulatory step in early lung development or amechanism whereby tumor cells possess the ability to down-regulate the expression of a more-differentiated phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 12:35:26