Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: Endothelial versus epithelial features
Autore:
Suda, K; Rothen-Rutishauser, B; Gunthert, M; Wunderli-Allenspach, H;
Indirizzi:
ETH Zurich, Dept Appl Biosci, Biopharm, CH-8057 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8057 pharm, CH-8057 Zurich, Switzerland
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
fascicolo: 8, volume: 37, anno: 2001,
pagine: 505 - 514
SICI:
1071-2690(200109)37:8<505:PCOHUV>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; IN-VITRO MODEL; ADHESION MOLECULE-1 EXPRESSION; INTERMEDIATE FILAMENT PROTEINS; P-GLYCOPROTEIN; BLADDER-CARCINOMA; MESOTHELIAL CELLS; LINE ECV304; MDCK CELLS; E-CADHERIN;
Keywords:
endothelia; epithelia; tight junction; cytoarchitecture; cell markers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Wunderli-Allenspach, H ETH Zurich, Dept Appl Biosci, Biopharm, Winterthurerstr 190, CH-8057 Zurich, Switzerland ETH Zurich Winterthurerstr 190 Zurich Switzerland CH-8057
Citazione:
K. Suda et al., "Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: Endothelial versus epithelial features", IN VITRO-AN, 37(8), 2001, pp. 505-514

Abstract

ECV304 cells reported as originating from human umbilical vein endothelialcells by spontaneous transformation have been used as a model cell line for endothelia over the last decade. Recently, deoxyribonucleic acid fingerprinting revealed an identical genotype for ECV304 and T24 cells (urinary bladder carcinoma cell line). In order to resolve the apparent discrepancy between the identical genotype and the fact that ECV304 cells phenotypically show important endothelial characteristics, a comparative study was performed. Immortalized porcine brain microvascular endothelial cells/C1-2, and Madin Darby canine kidney cells were included as typical endothelial and epithelial cells, respectively. Various methods, such as confocal laser scanningmicroscopy, Western blot, and protein activity tests, were used to study the cell lines. ECV304 and T24 cells differ in criteria, such as growth behavior, cytoarchitecture, tight junction arrangement, transmembrane electrical resistance, and activity of gamma -glutamyltransferase. Several endothelial markers (von Willebrand factor, uptake of low-density lipoprotein, vimentin) could clearly be identified in ECV304, but not in T24 cells. Desmoglein and cytokeratin, both known as epithelial markers, were found in ECV304 as well as in T24 cells. However, differences were found for the two cell lines with respect to the type of cytokeratin: in ECV304 cells mainly cytokeratin 18 (45 kDa) is found, whereas in T24 cells cytokeratin 8 (52 kDa) is predominant. As we could demonstrate, the ECV304 cell line exposes many endothelial features which, in view of the scarcity of suitable endothelial cell lines, still make it an attractive in vitro model for endothelia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 21:49:46