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Titolo:
Alterations induced by E-cadherin and beta-catenin antibodies during the development of Bufo arenarum (Anura-Bufonidae)
Autore:
Izaguirre, MF; Adur, JF; Soler, AP; Casco, VH;
Indirizzi:
Lankenau Inst Med Res, Wynnewood, PA USA Lankenau Inst Med Res Wynnewood PA USA u Inst Med Res, Wynnewood, PA USA Natl Univ Entre Rios, Sch Bioengn, Microscopy Lab, Oro Verde, Entre Rios, Argentina Natl Univ Entre Rios Oro Verde Entre Rios Argentina ntre Rios, Argentina
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 4, volume: 16, anno: 2001,
pagine: 1097 - 1106
SICI:
0213-3911(200110)16:4<1097:AIBEAB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CELL-ADHESION; EPIDERMAL GROWTH-FACTOR; TYROSINE PHOSPHORYLATION; XENOPUS-LAEVIS; MONOCLONAL-ANTIBODY; SIGNAL-TRANSDUCTION; MOLECULE UVOMORULIN; CYTOPLASMIC DOMAIN; EXPRESSION; IDENTIFICATION;
Keywords:
catenins; cadherins; morphogenesis; amphibians;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Casco, VH Natl Univ Entre Rios, Microscopy Lab, Sch Bioengn, CC 47,Suc 3, RA-1300 Entre Rios, Parana, Argentina Natl Univ Entre Rios CC 47,Suc 3 Entre Rios Parana Argentina RA-1300
Citazione:
M.F. Izaguirre et al., "Alterations induced by E-cadherin and beta-catenin antibodies during the development of Bufo arenarum (Anura-Bufonidae)", HIST HISTOP, 16(4), 2001, pp. 1097-1106

Abstract

E(epithelial)-cadherin is a member of a calcium-dependent family of cell surface glycoproteins involved in cell-cell adhesion and morphogenesis. Catenins are a large family of proteins that connect the cadherins to the cytoskeleton. They are important for cadherin function and for transducing signals involved in specification of cell fate during embryogenesis. The best characterized catenins include alpha-, beta-, gamma-, and p120-catenin. Usingspecific antibodies, we studied the expression and distribution of E-cadherin, and alpha- and beta -catenin in developmental stages of Bufo arenarum toad. The three proteins were found co-localized in stages 19 to 41 of development. Surprisingly, E-cadherin was the only of these three proteins found earlier than stage 19. To test whether E-cadherin and beta -catenin have a functional role in Bufo arenarum embryogenesis, stage 17 whole embryos were incubated with anti-E-cadherin and beta -catenin antibodies. Both anti-E-cadherin and anti-beta -catenin antibodies induced severe morphological alterations. However, while alterations produced by the anti-beta -catenin antibody, showed some variability from the most severe (neural tube and notochord duplication) to a simple delay in development, the alterations with anti-E-cadherin were homogeneous. These observations suggest a critical role for E-cadherin and beta -catenin in the early embryonic development of the Bufo arenarum toad. Our results are consistent with the developmental role of these proteins in other species. One of the most surprising findings wasthe blockage with the anti-beta -catenin antibodies on later embryo stages, and we hypothesize that the partial axes duplication could be mediated bythe notochord induction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:52:31