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Titolo:
Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes
Autore:
Breen, M; Jouquand, S; Renier, C; Mellersh, CS; Hitte, C; Holmes, NG; Cheron, A; Suter, N; Vignaux, F; Bristow, AE; Priat, C; McCann, E; Andre, C; Boundy, S; Gitsham, P; Thomas, R; Bridge, WL; Spriggs, HF; Ryder, EJ; Curson, A; Sampson, J; Ostrander, EA; Binns, MM; Galibert, F;
Indirizzi:
Fac Med, UMR 6061, CNRS, F-35043 Rennes, France Fac Med Rennes France F-35043 ed, UMR 6061, CNRS, F-35043 Rennes, France Anim Hlth Trust, Genet Sect, Newmarket CB8 7UU, Suffolk, England Anim HlthTrust Newmarket Suffolk England CB8 7UU 8 7UU, Suffolk, England Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98109 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98109 , Seattle, WA 98109 USA Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England Univ Leicester Leicester Leics England LE1 7RH er LE1 7RH, Leics, England
Titolo Testata:
GENOME RESEARCH
fascicolo: 10, volume: 11, anno: 2001,
pagine: 1784 - 1795
SICI:
1088-9051(200110)11:10<1784:CSFPAA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANIS-FAMILIARIS; LINKAGE MAP; HYBRID MAP; MUCOPOLYSACCHARIDOSIS-I; GENE; KARYOTYPE; CONSTRUCTION; MUTATION; MARKERS; MICROSATELLITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Breen, M Fac Med, UMR 6061, CNRS, F-35043 Rennes, France Fac Med Rennes France F-35043 061, CNRS, F-35043 Rennes, France
Citazione:
M. Breen et al., "Chromosome-specific single-locus FISH probes allow anchorage of an 1800-marker integrated radiation-hybrid/linkage map of the domestic dog genome to all chromosomes", GENOME RES, 11(10), 2001, pp. 1784-1795

Abstract

We present here the first fully integrated, comprehensive map of the canine genome, incorporating detailed cytogenetic, radiation hybrid (RH), and meiotic information. We have mapped a collection of 266 chromosome-specific cosmid clones, each containing a microsatellite marker, to all 38 canine autosomes by fluorescence in situ hybridization (FISH). A 1500-marker RH map, comprising 1078 microsatellites, 320 dog gene markers, and 102 chromosome-specific markers, has been constructed using the RHDF5000-2 whole-genome radiation hybrid panel. Meiotic linkage analysis was performed, with at least one microsatellite marker from each dog autosome on a panel of reference families, allowing one meiotic linkage group to be anchored to all 38 dog autosomes. We present a karyotype in which each chromosome is identified by one meiotic linkage group and one or more RH groups. This updated integrated map, containing a total of 1800 markers, covers > 90% of the dog genome. Positional selection of anchor clones enabled us, for the first time, to orientate nearly all of the integrated groups on each chromosome and to evaluate the extent of individual chromosome coverage in the integrated genome map. Finally, the inclusion of 320 dog genes into this integrated map enhancesexisting comparative mapping data between human and dog, and the 1000 mapped microsatellite markers constitute an invaluable tool with which to perform genome scanning studies on pedigrees of interest.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:13:14