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Titolo:
Assignment of 118 novel cDNAs of cynomolgus monkey brain to human chromosomes
Autore:
Osada, N; Hida, M; Kususda, J; Tanuma, R; Iseki, K; Hirata, M; Suto, Y; Hirai, M; Terao, K; Suzuki, Y; Sugano, S; Hashimoto, K;
Indirizzi:
Natl Inst Infect Dis, Div Genet Resources, Shinjuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis, Tsukuba Primate Ctr Med Sci, Tokyo, Japan Natl Inst Infect Dis Tokyo Japan kuba Primate Ctr Med Sci, Tokyo, Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Tokyo, Japan Univ Tokyo Tokyo Japan ontier Sci, Dept Integrated Biosci, Tokyo, Japan Univ Tokyo, Inst Med Sci, Dept Genome Struct Anal, Tokyo, Japan Univ Tokyo Tokyo Japan t Med Sci, Dept Genome Struct Anal, Tokyo, Japan Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 113, Japan Univ Tokyo Tokyo Japan 113 Grad Sch Sci, Dept Biol Sci, Tokyo 113, Japan
Titolo Testata:
GENE
fascicolo: 1, volume: 275, anno: 2001,
pagine: 31 - 37
SICI:
0378-1119(20010905)275:1<31:AO1NCO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; SEQUENCE; COMPLEXITY; NUCLEAR;
Keywords:
Macaca fascicularis; brain; full-length cDNA library; full insert sequence; fluorescent in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Osada, N Natl Inst Infect Dis, Div Genet Resources, Shinjuku Ku, 1-23-1 Toyama Cho,Tokyo 1628640, Japan Natl Inst Infect Dis 1-23-1 Toyama Cho TokyoJapan 1628640 Japan
Citazione:
N. Osada et al., "Assignment of 118 novel cDNAs of cynomolgus monkey brain to human chromosomes", GENE, 275(1), 2001, pp. 31-37

Abstract

In order to isolate genes that may not be represented in current human brain cDNA libraries, we have sequenced about 20,000 sequence tags of cDNA clones derived from cerebellum and parietal lobe of cynomolgus monkeys (Macacafascicularis). We determined the entire cDNA sequence of approximately 700clones whose 5'-terminal sequences showed no homology to annotated putative genes or expressed sequence tags in current databases of genetic information. From this, 118 clones with sequences encoding novel open reading frames of more than 100 amino acid residues were selected for further analysis. To localize the genes corresponding to these 118 newly identified cDNA clones on human chromosomes, we performed a homology search using the human genome sequence and fluorescent in situ hybridization. In total, 108 of 118 clones were successfully assigned to specific regions of human chromosomes. This result demonstrates that genes expressed in cynomolgus monkey are highly conserved throughout primate evolution, and that virtually all had human homologs. Furthermore, we will be able to discover novel human genes in thehuman genome using monkey homologs as probes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:15:00