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Titolo:
DRAMATIC ENHANCEMENT OF THE CATALYTIC ACTIVITY OF COAGULATION-FACTOR IXA BY ALCOHOLS
Autore:
STURZEBECHER J; KOPETZKI E; BODE W; HOPFNER KP;
Indirizzi:
UNIV JENA,ZENTRUM VASK BIOL & MED ERFURT,NORDHAUSER STR 78 D-99089 ERFURT GERMANY BOEHRINGER MANNHEIM GMBH,WERK PENZBERG D-82372 PENZBERG GERMANY MAX PLANCK INST BIOCHEM D-82152 MARTINSRIED GERMANY
Titolo Testata:
FEBS letters
fascicolo: 2, volume: 412, anno: 1997,
pagine: 295 - 300
SICI:
0014-5793(1997)412:2<295:DEOTCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA THROMBOPLASTIN ANTECEDENT; BOVINE FACTOR-IX; FACTOR-X; CHRISTMAS FACTOR; FACTOR-XIA; ACTIVATION; SITE;
Keywords:
COAGULATION FACTOR IXA; SYNTHETIC SUBSTRATE; CHROMOGENIC ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
J. Sturzebecher et al., "DRAMATIC ENHANCEMENT OF THE CATALYTIC ACTIVITY OF COAGULATION-FACTOR IXA BY ALCOHOLS", FEBS letters, 412(2), 1997, pp. 295-300

Abstract

The coagulation factor IXa (FIXa) exhibits a very weak proteolytic activity towards natural or synthetic substrates. Upon complex formationwith its cofactor FVIIIa and Ca2+-mediated binding to phospholipid membranes, FIXa becomes a very potent activator of FX, The presence of FVIIIa has no effect on the cleavage of peptide substrates by FIXa, however, We found that several alcohols dramatically enhance the catalytic activity of human FIXa towards synthetic substrates, Substrates withthe tripeptidyl moiety R-D-Xxx-Gly-Arg are especially susceptible to the enhanced FIXa catalysis. Maximal increase up to 20-fold has been measured in the presence of ethylene glycol. We suggest that alcohols modify the conformation of FIXa rendering the active-site cleft more easily accessible to tripeptide substrates with a hydrophobic residue inthe P3-position. (C) 1997 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:20:43