Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Engraftment of serotonergic precursors enhances locomotor function and attenuates chronic central pain behavior following spinal hemisection injury in the rat
Autore:
Hains, BC; Johnson, KM; McAdoo, DJ; Eaton, MJ; Hulsebosch, CE;
Indirizzi:
Univ Texas, Med Branch, Dept Anat & Neurosci, Galveston, TX 77555 USA UnivTexas Galveston TX USA 77555 nat & Neurosci, Galveston, TX 77555 USA Univ Texas, Med Branch, Inst Marine Biomed, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 Marine Biomed, Galveston, TX 77555 USA Univ Miami, Sch Med, Miami Project Cure Paralysis, Miami, FL 33136 USA Univ Miami Miami FL USA 33136 Project Cure Paralysis, Miami, FL 33136 USA
Titolo Testata:
EXPERIMENTAL NEUROLOGY
fascicolo: 2, volume: 171, anno: 2001,
pagine: 361 - 378
SICI:
0014-4886(200110)171:2<361:EOSPEL>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY AFFERENT-FIBERS; PRIMARY SENSORY NEURONS; NEUROTROPHIC FACTOR; CORD INJURY; MESSENGER-RNA; ADULT-RAT; MOTONEURON EXCITABILITY; NOXIOUS-STIMULATION; SPINOTHALAMIC TRACT; MONOSYNAPTIC REFLEX;
Keywords:
spinal cord injury; serotonin; brain-derived neurotrophic factor; transplantation; pain; locomotion; graft; transplant;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
108
Recensione:
Indirizzi per estratti:
Indirizzo: Hains, BC Univ Texas, Med Branch, Dept Anat & Neurosci, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 osci, Galveston, TX 77555 USA
Citazione:
B.C. Hains et al., "Engraftment of serotonergic precursors enhances locomotor function and attenuates chronic central pain behavior following spinal hemisection injury in the rat", EXP NEUROL, 171(2), 2001, pp. 361-378

Abstract

Spinal cord injury (SCI) results in abnormal locomotor and pain syndromes in humans. T13 spinal hemisection in the rat results in development of permanent mechanical allodynia and thermal hyperalgesia partially due to interruption of descending inhibitory modulators such as serotonin (5-HT). We hypothesize that lumbar transplantation of nonmitotic cells that tonically secrete antinociceptive and trophic compounds will reduce the pain-like behavior and enhance locomotor recovery after SCI We used RN46A-B14 cells, a conditionally immortalized (SV40tsTag) rat neuronal cell line derived from E13 raphe bioengineered to secrete both 5-HT and BDNF in vitro at both permissive (33 degreesC) and nonpermissive (39 degreesC) temperatures. Three groups(n = 72) of 30-day-old male Sprague-Dawley rats were spinally hemisected at T13 and allowed 4 weeks for adequate recovery of locomotor function and development of allodynia and hyperalgesia. Immunosuppressed animals receivedeither lumbar RN46A-B14 (n = 24) or control RN46A-V1 (n = 24) empty-vectortransplants or no cell (n = 24) transplant. HPLC analysis of media and CSFdemonstrated increases of both in vitro and in vivo 5-HT levels at 28 daysin RN46A-B14 animals. ELISA demonstrated BDNF secretion in vitro and in vivo by RNA46A-B14 cells Locomotor function (BBB scale) and nociceptive behaviors measured by paw withdrawals to von Frey filaments, radiant heat, and noxious pin stimuli were tested for 4 weeks posttransplant. Animals receiving RN46A-B14 cells demonstrated significantly improved locomotor function and reductions in both fore- and hindlimb mechanical allodynia and thermal hyperalgesia compared to controls receiving RN46A-V1 or no transplants. Theseeffects were modulated by the 5-HT antagonist methysergide and reuptake inhibitor fluvoxamine. Bromodeoxyuridine and 5-HT immunoreactivity confirmed cell survival and graft location 4 weeks posttransplantation. These resultssupport the therapeutic potential of bioengineered serotonin-secreting cell lines in reducing chronic central pain following spinal cord injury. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 02:13:57