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Titolo:
Efficient lentiviral transduction of human cord blood CD34(+) cells followed by their expansion and differentiation into dendritic cells
Autore:
Oki, M; Ando, K; Hagihara, M; Miyatake, H; Shimizu, T; Miyoshi, H; Nakamura, Y; Matsuzawa, H; Sato, T; Ueda, Y; Gansuvd, B; Kato, S; Hotta, T;
Indirizzi:
Tokai Univ, Sch Med, Dept Med, Div Hematol & Rheumatol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 l & Rheumatol, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Pediat, Res Ctr Genet Engn & Cell Transplantat, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 Transplantat, Kanagawa 2591193, Japan Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, Tsukuba, Ibaraki, Japan Univ Tsukuba Tsukuba Ibaraki Japan Dept Immunol, Tsukuba, Ibaraki, Japan
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 10, volume: 29, anno: 2001,
pagine: 1210 - 1217
SICI:
0301-472X(200110)29:10<1210:ELTOHC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; COLONY-STIMULATING FACTOR; SERUM-FREE CONDITIONS; VERSUS-HOST DISEASE; LONG-TERM CULTURES; EX-VIVO EXPANSION; STEM-CELLS; IN-VITRO; REPOPULATING CELLS; PROGENITOR CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ando, K Tokai Univ, Sch Med, Dept Med, Div Hematol & Rheumatol, Kanagawa 2591193, Japan Tokai Univ Kanagawa Japan 2591193 matol, Kanagawa 2591193, Japan
Citazione:
M. Oki et al., "Efficient lentiviral transduction of human cord blood CD34(+) cells followed by their expansion and differentiation into dendritic cells", EXP HEMATOL, 29(10), 2001, pp. 1210-1217

Abstract

Objective. To support immune reconstitution after cord blood transplantation, immunotherapy using gene-modified dendritic cells (DCs), the most potent antigen-presenting cells, can be a powerful strategy for preventing infection and recurrence. To investigate the applicability of lentiviral vector-transduced DCs compared to retroviral vectors, we transduced umbilical cordblood (CB) CD34(+) cells, then expanded and differentiated them into DCs. Materials and Methods. We transduced CB CD34(+) cells by vesicular stomatitis virus G-protein pseudotyped self-inactivating lentiviral vector or retroviral vectors carrying the enhanced green fluorescent protein gene. The cells were expanded in the stroma-dependent culture system and transferred tothe culture condition for developing DCs. The efficiency of transduction and expression of the transgene in severe combined immunodeficiency (SCID) mice-repopulating cells (SRCs) and DCs were compared between lentiviral vector and retroviral vectors. Induced DCs were cocultured with allogeneic or autologous T cells to test the ability to present antigens. Results. CB CD34(+) cells transduced by lentiviral vector and expanded ex vivo sustained stable transgene expression and multipotentiality by assessing SRCs assay and clonogenic assay of bone marrow cells from the transplanted mice. DCs derived from these cells expressed green fluorescent protein and surface markers CD1a, CD80, and HLA-DR and showed potent allo-stimulatory activity as well as nontransduced DCs did. On the other hand, we did not detect transgene expression in SRCs and DCs transduced by retroviral vectors. Conclusion. Gene-modified DCs derived from ex vivo expanded CB CD34(+) cells transduced by lentiviral vector will be useful in future immunotherapy protocols. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 02/04/20 alle ore 02:50:44