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Titolo:
Loss of E-cadherin expression in gastric intestinal metaplasia and later stage p53 altered expression in gastric carcinogenesis
Autore:
Ma, MC; Devereux, TR; Stockton, P; Sun, KL; Sills, RC; Clayton, N; Portier, M; Flake, G;
Indirizzi:
NIEHS, Lab Expt Pathol, NIH, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ol, NIH, Res Triangle Pk, NC 27709 USA NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA China Med Univ, Dept Mol Genet, Shenyang, Peoples R China China Med Univ Shenyang Peoples R China enet, Shenyang, Peoples R China
Titolo Testata:
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
fascicolo: 4, volume: 53, anno: 2001,
pagine: 237 - 246
SICI:
0940-2993(200109)53:4<237:LOEEIG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELICOBACTER-PYLORI INFECTION; TRANSCRIPTION FACTOR LEF-1; GENE-MUTATIONS PROVIDE; NITRIC-OXIDE SYNTHASE; BETA-CATENIN; DNA HYPERMETHYLATION; COLORECTAL-CANCER; BREAST-CANCER; CELL-LINES; CARCINOMAS;
Keywords:
E-cadherin; intestinal metaplasia; p53; gastric carcinogenesis; Helicobacter pylori; hypermethylation; beta-catenin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Flake, G NIEHS, Lab Expt Pathol, NIH, MD B3-06,POB 12233, Res Triangle Pk,NC 27709USA NIEHS MD B3-06,POB 12233 Res Triangle Pk NC USA 27709 NC 27709USA
Citazione:
M.C. Ma et al., "Loss of E-cadherin expression in gastric intestinal metaplasia and later stage p53 altered expression in gastric carcinogenesis", EXP TOX PAT, 53(4), 2001, pp. 237-246

Abstract

Gastric cancers are commonly subdivided into intestinal and diffuse subtypes on a morphologic basis, supported by corollary evidence of differences at the pathogenetic and molecular levels. Chronic atrophic gastritis with intestinal metaplasia is a common precursor lesion for the intestinal type ofcarcinoma. To identify early molecular changes, in this study we have examined 13 surgical specimens both for the expression of E-cadherin, p53 and beta -catenin by immunohistochemistry and for methylation of the CDHI promoter (E-cadherin) by bisulfite genomic sequencing of laser capture microdissected samples. Each specimen examined contained areas of normal (nonmetaplastic) gastric mucosa, as well as areas of intestinal metaplasia and/or carcinoma. Reduced or absent E-cadherin and partial to complete methylation of one to multiple CpG sites examined in the CDHI promoter were observed in allof the metaplasia samples. Thus, the methylation status of the CDHI promoter and expression of E-cadherin together provide strong evidence that loss of E-cadherin is an early event in intestinal type gastric carcinogenesis. In contrast, expression of p53, assumed to be mutant p53, was generally notdetected (except for isolated cells) until the carcinoma stage in tissues from these patients. These results suggest that mutation of p53 is a late event in intestinal type gastric cancer. The level of beta -catenin expression did not appear to change between normal, metaplastic and carcinoma cellsof intestinal type, and no nuclear staining was visible in any of the tissues. These results suggest that the Wnt signaling pathway is not upregulated in this type of cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:46:51