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Titolo:
Effect of vitamin E and vitamin C on the DNA synthesis of human umbilical arterial endothelial cells
Autore:
Totzke, G; Metzner, C; Ulrich-Merzenich, G; Ko, Y; Sachinidis, A; Vetter, H;
Indirizzi:
Univ Bonn, Med Poliklin, D-53111 Bonn, Germany Univ Bonn Bonn Germany D-53111 Bonn, Med Poliklin, D-53111 Bonn, Germany
Titolo Testata:
EUROPEAN JOURNAL OF NUTRITION
fascicolo: 3, volume: 40, anno: 2001,
pagine: 121 - 126
SICI:
1436-6207(200106)40:3<121:EOVEAV>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; SMOOTH-MUSCLE CELL; ALPHA-TOCOPHEROL; INHIBITION; CULTURE; ATHEROSCLEROSIS; PROLIFERATION; PATHOGENESIS; OXIDATION; ANTIOXIDANTS;
Keywords:
vitamin E; vitamin C; DNA synthesis; human endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Vetter, H Univ Bonn, Med Poliklin, Wilhelmstr 35-37, D-53111 Bonn, GermanyUniv Bonn Wilhelmstr 35-37 Bonn Germany D-53111 1 Bonn, Germany
Citazione:
G. Totzke et al., "Effect of vitamin E and vitamin C on the DNA synthesis of human umbilical arterial endothelial cells", EUR J NUTR, 40(3), 2001, pp. 121-126

Abstract

Background Endothelial cell growth and reendothealization after vascular injury protect the vessel wall against endothelial dysfunction which is believed to play a major role in the pathogenesis of atherosclerosis. Aim of the study To investigate a possible protective role of antioxidant vitamins in the present study, the effect of vitamin E (alpha -tocopherol) alone and in combination with vitamin C on the DNA synthesis of human umbilical arterial endothelial cells (HUAEC) was examined. Furthermore, because oxidized low-density lipoprotein (ox-LDL) is thought to be involved in atherogenesis,the combined effect of vitamin E and vitamin C with ox-LDL and the influence of vitamin-pretreated LDL on HUAEC proliferation were investigated. Methods DNA-synthesis was determined by measurement of [H-3]thymidine incorporation into the cell DNA. Results Vitamin E alone and in combination with vitamin C resulted in an increase in [H-3]thymidine incorporation into cell DNA, especially in the presence of basic fibroblast growth factor (bFGF). Allvitamin-pretreated LDL samples and ox-LDL led to a nearly complete inhibition of endothelial DNA-synthesis. The ox-LDL-induced effect could not be prevented by vitamin E alone nor in combination with vitamin C. Conclusions It seems that once LDL oxidation is in process, vitamin E alone and in combination with vitamin C is ineffective to exert its antioxidative capacity under the conditions used. Thus, vitamin E alone and combined with vitamin C may act as antiatherogens by inducing endothelial cell growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:26:12