Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Developmental neurotoxicity of chlorpyrifos modeled in vitro: Comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesisin PC12 and C6 cells
Autore:
Qiao, D; Seidler, FJ; Slotkin, TA;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 9, volume: 109, anno: 2001,
pagine: 909 - 913
SICI:
0091-6765(200109)109:9<909:DNOCMI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; IN-VITRO; ADULT-RATS; ACETYLCHOLINESTERASE INHIBITION; PHEOCHROMOCYTOMA CELLS; MUSCARINIC RECEPTORS; GESTATIONAL EXPOSURE; CELLULAR MECHANISMS; BRAIN; VIVO;
Keywords:
C6 cells; chlorpyrifos; developmental neurotoxicity; diazinon; DNA synthesis; PC12 cells; physostigmine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Slotkin, TA Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Box 3813, Durham, NC 27710USA Duke Univ Box 3813 Durham NC USA 27710 13, Durham, NC 27710USA
Citazione:
D. Qiao et al., "Developmental neurotoxicity of chlorpyrifos modeled in vitro: Comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesisin PC12 and C6 cells", ENVIR H PER, 109(9), 2001, pp. 909-913

Abstract

The widely used organophosphate pesticide chlorpyrifos is a suspected neuroteratogen. In the current study, we compared the effects of chlorpyrifos and its major metabolites in two in vitro models, neuronotypic PC12 cells and gliotypic C6 cells. Chlorpyrifos inhibited DNA synthesis in both cell lines but had a greater effect on gliotypic cells. Chlorpyrifos oxon, the active metabolite that inhibits cholinesterase, also decreased DNA synthesis inPC12 and C6 cells with a preferential effect on the latter. Trichloropyridinol, the major catabolic product of chlorpyrifos, had a much smaller, but nevertheless statistically significant, effect that was equivalent in both cell lines. Diazinon, another organophosphate pesticide, also inhibited DNAsynthesis with preference toward C6 cells, but was less effective than waschlorpyrifos. Physostigmine, a non-organophosphate cholinesterase inhibitor, was less effective than either chlorpyrifos or diazinon, but still caused significant inhibition of DNA synthesis in C6 cells. We also found that the addition of sera protected the cells from the adverse effects of chlorpyrifos and that the effect could be reproduced by addition of albumin. Theseresults indicate that chlorpyrifos and other organophosphates such as diazinon have immediate, direct effects on neural cell replication, preferentially for gliotypic cells. In light of the protective effect of serum proteins, the fact that the fetus and newborn possess lower concentrations of these proteins suggests that greater neurotoxic effects may occur at blood levels of chlorpyrifos that are nontomc to adults.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:40:22