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Titolo:
Design and development of a catalytic ribonucleoprotein
Autore:
Atsumi, S; Ikawa, Y; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
EMBO JOURNAL
fascicolo: 19, volume: 20, anno: 2001,
pagine: 5453 - 5460
SICI:
0261-4189(20011001)20:19<5453:DADOAC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; TRANSFER-RNA SYNTHETASE; TETRAHYMENA RIBOZYME; TERTIARY STRUCTURE; BACTERIOPHAGE-LAMBDA; ALPHA-HELIX; PROTEIN; RECOGNITION; COMPLEX; ELEMENT;
Keywords:
group I intron; in vivo selection; ribonucleoprotein; ribozyme; RNA-protein interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Sci, Kyoto 6068502, Japan Kyoto Univ KyotoJapan 6068502 ad Sch Sci, Kyoto 6068502, Japan
Citazione:
S. Atsumi et al., "Design and development of a catalytic ribonucleoprotein", EMBO J, 20(19), 2001, pp. 5453-5460

Abstract

Ribonucleoproteins (RNPs) consisting of derivatives of a ribozyme and an RNA-binding protein were designed and constructed based upon high-resolutionstructures of the corresponding prototype molecules, the Tetrahymena groupI self-splicing intron RNA and two proteins (bacteriophage lambdaN and HIVRev proteins) containing RNA-binding motifs. The splicing reaction proceeds efficiently only when the designed RNA associates with the designed protein either in vivo or in vitro. In vivo mutagenic protein selection was effective for improving the capability of the protein. Kinetic analyses indicate that the protein promotes RNA folding to establish an active conformation. The fact that the conversion of a ribozyme to an RNP can be accomplished by simple molecular design supports the RNA world hypothesis and suggests that a natural active RNP might have evolved readily from a ribozyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 15:01:11