Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
XMeis3 protein activity is required for proper hindbrain patterning in Xenopus laevis embryos
Autore:
Dibner, C; Elias, S; Frank, D;
Indirizzi:
Technion Israel Inst Technol, Rappaport Family Inst Res Med Sci, Dept Biochem, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 L-31096 Haifa, Israel
Titolo Testata:
DEVELOPMENT
fascicolo: 18, volume: 128, anno: 2001,
pagine: 3415 - 3426
SICI:
0950-1991(200109)128:18<3415:XPAIRF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; BONE MORPHOGENETIC PROTEIN-4; RETINOIC ACID; SPEMANN ORGANIZER; NEURAL TISSUE; NOGGIN; DIFFERENTIATION; EXPRESSION; INDUCTION; POSTERIOR;
Keywords:
Xenopus laevis; XMeis3; antimorph; antisense morpholino oligonucleotides; caudalization; hindbrain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Frank, D Technion Israel Inst Technol, Rappaport Family Inst Res Med Sci, Dept Biochem, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 aifa, Israel
Citazione:
C. Dibner et al., "XMeis3 protein activity is required for proper hindbrain patterning in Xenopus laevis embryos", DEVELOPMENT, 128(18), 2001, pp. 3415-3426

Abstract

Meis-family homeobox proteins have been shown to regulate cell fate specification in vertebrate and invertebrate embryos. Ectopic expression of RNA encoding the Xenopus Meis3 (XMeis3) protein caused anterior neural truncations with a concomitant expansion of hindbrain and spinal cord markers in Xenopus embryos. In naive animal cap explants, XMeis3 activated expression of posterior neural markers in the absence of pan-neural markers. Supporting its role as a neural caudalizer, XMeis3 is expressed in the hindbrain and spinal cord. We show that XMeis3 acts like a transcriptional activator, and its caudalizing effects can be mimicked by injecting RNA encoding a VP16-XMeis3 fusion protein. To address the role of endogenous XMeis3 protein in neural patterning, XMeis3 activity was antagonized by injecting RNA encoding an Engrailed-XMeis3 antimorph fusion protein or XMeis3 antisense morpholino oligonucleotides. In these embryos, anterior neural structures were expanded and posterior neural tissues from the midbrain-hindbrain junction throughthe hindbrain were perturbed. In neuralized animal cap explants, XMeis-antimorph protein modified caudalization by basic fibroblast growth factor andWnt3a. XMeis3-antimorph protein did not inhibit caudalization per se, but re-directed posterior neural marker expression to more anterior levels; it reduced expression of spinal cord and hindbrain markers, yet increased expression of the more rostral En2 marker. These results provide evidence that XMeis3 protein in the hindbrain is required to modify anterior neural-inducing activity, thus, enabling the transformation of these cells to posteriorfates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:43:18