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Titolo:
Expression of basic helix-loop-helix proteins in the glomeruli
Autore:
Imabayashi, T; Iehara, N; Takeoka, H; Uematsu-Yanagita, M; Kataoka, H; Nishikawa, S; Sano, H; Yokode, M; Fukatsu, A; Kita, T; Doi, T;
Indirizzi:
Univ Tokushima, Sch Med, Dept Lab Med, Tokushima 770, Japan Univ Tokushima Tokushima Japan 770 d, Dept Lab Med, Tokushima 770, Japan Kyoto Univ, Fac Med, Div Clin Bioregulatory Sci, Kyoto, Japan Kyoto Univ Kyoto Japan ac Med, Div Clin Bioregulatory Sci, Kyoto, Japan Kyoto Univ, Fac Med, Dept Mol Genet, Kyoto, Japan Kyoto Univ Kyoto Japan yoto Univ, Fac Med, Dept Mol Genet, Kyoto, Japan Kyoto Univ, Fac Med, Div Artificial Kidneys, Kyoto, Japan Kyoto Univ Kyoto Japan v, Fac Med, Div Artificial Kidneys, Kyoto, Japan
Titolo Testata:
CLINICAL NEPHROLOGY
fascicolo: 1, volume: 55, anno: 2001,
pagine: 53 - 58
SICI:
0301-0430(200101)55:1<53:EOBHPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MESANGIAL CELLS; SMOOTH-MUSCLE; NEGATIVE REGULATOR; END-PRODUCTS; MYOD FAMILY; ALPHA; ACTIN; GENES;
Keywords:
basic helix-loop-helix; mesangial cells; myf5; herculin; Id;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Doi, T Univ Tokushima, Sch Med, Dept Lab Med, 3-18-15 Kuramoto Cho, Tokushima 770, Japan Univ Tokushima 3-18-15 Kuramoto Cho Tokushima Japan 770 770, Japan
Citazione:
T. Imabayashi et al., "Expression of basic helix-loop-helix proteins in the glomeruli", CLIN NEPHR, 55(1), 2001, pp. 53-58

Abstract

Background: Basic helix loop helix (bHLH) proteins play a critical role inthe differentiation of not only striated muscle cells but also adipocytes,neuron cells and smooth muscle cells, Previous studies have established invitro mouse mesangial cells (MCs) to maintain the differentiated smooth muscle phenotype. Materials and methods: The purpose of the present study wasto clone bHLH proteins from these MCs using the primers designed from a homologous sequence specific to bHLH, and to analyze the presence of bHLH proteins in normal kidney in vivo. From the cloning of MCs in vitro, we identified myf5 and herculin mRNA but not myoD. The expression of bHLH proteins in vivo was examined by immunohistochemistry with each specific antibody. Results: The MCs in newborn mice possessed Id but did not express either protein herculin or myoD. On the other hand, mature MCs expressed both myf5 andherculin. The Id protein disappeared in mature glomeruli. Conclusion: These results suggest that bHLH proteins are an important factor for mature MCsin vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:16:13