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Titolo:
Matrilysin mediates extracellular cleavage of E-cadherin from prostate cancer cells: A key mechanism in hepatocyte growth factor/scatter factor-induced cell-cell dissociation and in vitro invasion
Autore:
Davies, G; Jiang, WG; Mason, MD;
Indirizzi:
Univ Wales Coll Med, Dept Surg, Metastasis Res Grp, Cardiff CF14 4XN, S Glam, Wales Univ Wales Coll Med Cardiff S Glam Wales CF14 4XN CF14 4XN, S Glam, Wales Velindre Hosp, Dept Med, Sect Clin Oncol, Cardiff CF4 7XL, S Glam, Wales Velindre Hosp Cardiff S Glam Wales CF4 7XL Cardiff CF4 7XL, S Glam, Wales
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 10, volume: 7, anno: 2001,
pagine: 3289 - 3297
SICI:
1078-0432(200110)7:10<3289:MMECOE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLUBLE ADHESION MOLECULES; FACTOR SCATTER FACTOR; MATRIX METALLOPROTEINASES; BLADDER-CANCER; BETA-CATENIN; INTERCELLULAR-ADHESION; CLINICAL IMPLICATIONS; ESOPHAGEAL CANCER; GASTRIC-CANCER; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Davies, G Univ Wales Coll Med, Dept Surg, Metastasis Res Grp, Hlth Pk, Cardiff CF14 4XN, S Glam, Wales Univ Wales Coll Med Hlth Pk Cardiff S Glam Wales CF14 4XN Wales
Citazione:
G. Davies et al., "Matrilysin mediates extracellular cleavage of E-cadherin from prostate cancer cells: A key mechanism in hepatocyte growth factor/scatter factor-induced cell-cell dissociation and in vitro invasion", CLIN CANC R, 7(10), 2001, pp. 3289-3297

Abstract

Purpose: The current study examined the effects of hepatocyte growth factor/scatter factor (HGF/SF) on cell-cell dissociation, invasion, and its association with the mediated release of matrix metalloproteinase-7 (Matrilysin) on the extracellular cleavage of E-cadherin in prostate cancer cells. Experimental Design: The effects of HGF/SF on cell-cell dissociation, in vitro invasion, and on the expression of E-cadherin at both protein and mRNAlevels were assessed in cells whose expression of Matrilysin was altered by treatment with antisense oligonucleotide. Results: Incubation with HGF/SF mediated the release of active Matrilysin (M-r 19,000), resulting in extracellular cleavage of E-cadherin from prostate cancer cells. This resultant soluble M-r 80,000 fragment of E-cadherin was subsequently recognized upon immunoprobing with an anti-E-cadherin antibody. Both recombinant human Matrilysin (rh-Matrilysin) and/or HGF/SF increased the level of soluble E-cadherin and decreased the level of full-length (M-r 120,000) E-cadherin as detected by Western blotting. The effects of rh-Matrilysin and HGF/SF were inhibited by an antisense oligonucleotide specifically directed toward human Matrilysin. In addition, stimulation with either rh-Matrilysin or HGF/SF resulted in disruption to the E-cadherin/beta -catenin complex, as shown by a significant increase (P < 0.05) in both cellscattering and invasion index. Conclusions: Treatment with HGF/SF induced Matrilysin-mediated cleavage tothe extracellular domain of E-cadherin, resulting in its dissociation fromthe cadherin/ catenin complex. This provides a new mechanism in HGF/ SF-induced cell scattering, resulting in a switch to a more invasive phenotype in LNCapFGC cells, as demonstrated by in vitro invasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:05:57