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Titolo:
Quantitation of soluble and skeletal alkaline phosphatase, and insoluble alkaline phosphatase anchor-hydrolase activities in human serum
Autore:
Anh, DJ; Eden, A; Farley, JR;
Indirizzi:
Jerry L Pettis Mem Vet Adm Med Ctr, Loma Linda, CA 92357 USA Jerry L Pettis Mem Vet Adm Med Ctr Loma Linda CA USA 92357 , CA 92357 USA Loma Linda Univ, Dept Med, Loma Linda, CA 92357 USA Loma Linda Univ Loma Linda CA USA 92357 ept Med, Loma Linda, CA 92357 USA Loma Linda Univ, Dept Biochem, Loma Linda, CA 92357 USA Loma Linda Univ Loma Linda CA USA 92357 Biochem, Loma Linda, CA 92357 USA
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 2, volume: 311, anno: 2001,
pagine: 137 - 148
SICI:
0009-8981(20010925)311:2<137:QOSASA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPASE-D ACTIVITY; LIVER PLASMA-MEMBRANES; GLYCOSYL-PHOSPHATIDYLINOSITOL; INFANTILE HYPOPHOSPHATASIA; HEAT INACTIVATION; GROWTH-FACTORS; BONE-FORMATION; PROTEINS; CELLS; PURIFICATION;
Keywords:
ALP anchor-hydrolase; human serum; anchorless form;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Farley, JR Jerry L Pettis Mem Vet Adm Med Ctr, 11201 Benton St, Loma Linda, CA 92357 USA Jerry L Pettis Mem Vet Adm Med Ctr 11201 Benton St Loma Linda CA USA 92357
Citazione:
D.J. Anh et al., "Quantitation of soluble and skeletal alkaline phosphatase, and insoluble alkaline phosphatase anchor-hydrolase activities in human serum", CLIN CHIM A, 311(2), 2001, pp. 137-148

Abstract

Background: The current studies were intended to compare the circulating levels of total and anchorless (soluble) skeletal and hepatic ALP isoenzyme activities, and insoluble ALP anchor- hydrolase activity in serum of postmenopausal women. Methods: Preliminary studies of the insoluble ALP anchor-hydrolase activity in serum revealed a pH Optimum of pH 5-6.5, a sensitivity to inactivation by heat at temperatures > 45 degreesC (t(1/2) = 8-9 min at 60 degreesC) and an apparent K-M (at pH 7.5) of 40-45 mU/ml of insoluble skeletal ALP activity. Results: Serum analyses showed that 94.5 +/- 0.5% (mean SEM) of the ALP activity in serum was in the anchorless, soluble form. The data were also consistent with the notion that the amount of insoluble ALP anchor- hydrolase activity in serum, 52.8 +/- 0.8 U/l (mean SEM), was sufficient for the conversion of anchor-intact (insoluble) ALP into the anchorless, soluble form, assuming activation by serum lipids and/or bile salts. Distributions of results for total. skeletal, hepatic, and insoluble ALP anchor-hydrolase activity were skewed toward the higher range and leptokurtotic (p < 0.01 for each). Total ALP activity ranged from 42% to 208% of the group mean value: skeletal, hepatic, and insoluble ALP anchor-hydrolase activities ranged from 5% to 306%, 33% to 277%, and 2% to 325%, respectively. In contrast. the soluble ALP fraction only ranged from 71% to 106% of the group mean value. Conclusions: The correlations between the total and both skeletal (r = 0.711, p < 0.001) and hepatic (r = 0.782, p < 0,001) ALP isoform activities were predictive. Although correlations were also observed between insoluble ALP anchor-hydrolase activity and total (r = 0.197, p < 0.001), hepatic (r = 0,184, p < 0.001) and skeletal ALP activities (r = 0.118, p< 0.05), those relationships were not predictive (r(2) < 0.04). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 07:43:18