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Titolo:
Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract
Autore:
Lu, ZG; Yang, W; Cao, Q; Tao, W; Hu, JC; Zhai, ZH;
Indirizzi:
Beijing Univ, Coll Life Sci, Beijing 100871, Peoples R China Beijing UnivBeijing Peoples R China 100871 jing 100871, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 17, volume: 46, anno: 2001,
pagine: 1449 - 1453
SICI:
1001-6538(200109)46:17<1449:IOIOAS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CASPASE-ACTIVATED DNASE; CELL-FREE APOPTOSIS; FRAGMENTATION FACTOR; REQUIREMENT; DEGRADATION; PROTEIN;
Keywords:
Xenopus laevis; apoptosis; IXAD; XAD; DNA Ladder; cytochrome c; cell-free system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Zhai, ZH Beijing Univ, Coll Life Sci, Beijing 100871, Peoples R China Beijing Univ Beijing Peoples R China 100871 71, Peoples R China
Citazione:
Z.G. Lu et al., "Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract", CHIN SCI B, 46(17), 2001, pp. 1449-1453

Abstract

When added with cytochrome c, Xenopus laevis egg extract XS-150 can induceexogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with NAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that MAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:17:37