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Titolo:
NF-kappa B and the MAP kinases/AP-1 pathways are both involved in interleukin-6 and interleukin-8 expression in fibroblast-like synoviocytes stimulated by protein I/II, a modulin from oral streptococci
Autore:
Neff, L; Zeisel, M; Sibilia, J; Scholler-Guinard, M; Klein, JP; Wachsmann, D;
Indirizzi:
Univ Strasbourg 1, Fac Pharm, INSERM, U392,Lab Immunol & Biochim Bacterienne, F-67400 Illkirch Graffenstaden, France Univ Strasbourg 1 Illkirch Graffenstaden France F-67400 enstaden, France Hop Univ Strasbourg, Dept Rheumatol, Strasbourg, France Hop Univ Strasbourg Strasbourg France ept Rheumatol, Strasbourg, France
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 10, volume: 3, anno: 2001,
pagine: 703 - 712
SICI:
1462-5814(200110)3:10<703:NBATMK>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MUTANS SR PROTEIN; VIRIDANS STREPTOCOCCI; RHEUMATOID-ARTHRITIS; ENDOTHELIAL-CELLS; SIGNAL-TRANSDUCTION; TRANSCRIPTION FACTORS; CYTOKINE PRODUCTION; INHIBITORY FACTOR; EPITHELIAL-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Wachsmann, D Univ Strasbourg 1, Fac Pharm, INSERM, U392,Lab Immunol & Biochim Bacterienne, 74 Route Rhin, F-67400 Illkirch Graffenstaden, France UnivStrasbourg 1 74 Route Rhin Illkirch Graffenstaden France F-67400
Citazione:
L. Neff et al., "NF-kappa B and the MAP kinases/AP-1 pathways are both involved in interleukin-6 and interleukin-8 expression in fibroblast-like synoviocytes stimulated by protein I/II, a modulin from oral streptococci", CELL MICROB, 3(10), 2001, pp. 703-712

Abstract

As in rheumatoid arthritis (RA), it was demonstrated recently that bacterial fragments of DNA or rRNA are present in the joint and therefore could play a role in inducing or perpetuating the disease, this work was initiated to define mechanisms that account for the stimulatory activities of the oral streptococcal modulin, protein I/II, on fibroblast-like synoviocytes (FLSs) from RA patients. FLSs from RA patients were stimulated with protein I/II, and expression of interleukin (IL)-6 and IL-8 mRNA was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Immunoblotting by antibodies specific for activated forms of MAPKs and electrophoretic mobility shift assays (EMSAs) were performed to study downstream signalling, which allowed the synthesis of IL-6 and IL-8. We reported that protein I/II interactions with FLSs from RA patients trigger the synthesis and release of IL-6and IL-8. We also demonstrated that protein I/II enhances the phosphorylation of ERK 1/2, p38 and JNKs and that ERK 1/2 and JNK MAPKs seem to play a more important role than p38 in protein I/II-mediated synthesis of IL-6 andIL-8. Our experiments also indicated that stimulation of FLSs with proteinI/II induces nuclear translocation of NF-kappaB, AP-1-binding activity andthat NF-kappaB plays a major role in IL-6 and IL-8 secretion from activated cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:13:30