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Titolo:
Cell cycle distribution of primitive haematopoietic cells stimulated in vitro and in vivo
Autore:
Zhang, XW; Audet, J; Piret, JM; Li, YX;
Indirizzi:
Univ British Columbia, Dept Math, Vancouver, BC V6T 1Z2, Canada Univ British Columbia Vancouver BC Canada V6T 1Z2 ver, BC V6T 1Z2, Canada Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1W5, Canada Univ British Columbia Vancouver BC Canada V6T 1W5 ver, BC V6T 1W5, Canada Univ British Columbia, Dept Biol & Chem Engn, Vancouver, BC V5Z 1M9, Canada Univ British Columbia Vancouver BC Canada V5Z 1M9 ver, BC V5Z 1M9, Canada
Titolo Testata:
CELL PROLIFERATION
fascicolo: 5, volume: 34, anno: 2001,
pagine: 321 - 330
SICI:
0960-7722(200110)34:5<321:CCDOPH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; EXPANSION IN-VITRO; HUMAN BONE-MARROW; DIVISION TRACKING; PROGENITOR CELLS; CULTURES; KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Li, YX Univ British Columbia, Dept Math, Math Annex 1202, Vancouver, BC V6T 1Z2, Canada Univ British Columbia Math Annex 1202 Vancouver BC Canada V6T1Z2 a
Citazione:
X.W. Zhang et al., "Cell cycle distribution of primitive haematopoietic cells stimulated in vitro and in vivo", CELL PROLIF, 34(5), 2001, pp. 321-330

Abstract

A novel approach is used to study the proliferating behaviour of primitivehaematopoietic cell populations in response to different stimuli. A mathematical model based on the average proportion of apoptotic, dividing and quiescent cells in primitive haematopoietic cell populations is developed to describe the mitotic history of 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester-labelled cells. The cell cycle distributions in different cytokine-supplemented cultures of primitive human and mouse bone marrow cells are determined and compared with those found in vivo. The results indicate that a combination of flt-3 ligand, Steel factor and interleukin-11 or hyper-interleukin-6 provide a level of mitogenic stimulation similar to that existing in vivo after a myeloablative radiation dose. The comparison of the cell cycle distribution obtained for different cultures of human bone marrow CD34(+)(45RA/71)(-) cells demonstrates that the addition of flt-3 ligand in these cultures decreases apoptosis significantly but does not reduce quiescence. In addition, in vivo and in vitro, it was found that more than 3days of stimulation are required to recruit a maximum number of quiescent cells into active cell cycle. These kinetics of cell cycle activation are found to be similar to those identified for the haematopoietic stem cells compartment in the same cultures. This mathematical analysis provides a useful tool for the development of haematopoietic stem cell culture processes for clinical applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:17:00