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Titolo:
Cell volume and rate of proliferation, but not protein expression pattern,distinguish pup/intimal smooth muscle cells from subcultured adult smooth muscle cells
Autore:
McKilligin, E; Grainger, DJ;
Indirizzi:
Addenbrookes Hosp, Dept Med, Cambridge CB2 2QQ, England Addenbrookes HospCambridge England CB2 2QQ , Cambridge CB2 2QQ, England
Titolo Testata:
CELL PROLIFERATION
fascicolo: 5, volume: 34, anno: 2001,
pagine: 275 - 292
SICI:
0960-7722(200110)34:5<275:CVAROP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC PLAQUES; PHENOTYPE; DIFFERENTIATION; RESTENOSIS; FEATURES; ARTERIES; CULTURE; LESIONS; SERUM; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Grainger, DJ Addenbrookes Hosp, Dept Med, Box 157,Hills Rd, Cambridge CB2 2QQ, England Addenbrookes Hosp Box 157,Hills Rd Cambridge England CB2 2QQ
Citazione:
E. McKilligin e D.J. Grainger, "Cell volume and rate of proliferation, but not protein expression pattern,distinguish pup/intimal smooth muscle cells from subcultured adult smooth muscle cells", CELL PROLIF, 34(5), 2001, pp. 275-292

Abstract

Smooth muscle cells from neonatal rats and from injured blood vessels growwith a characteristic cobblestone morphology that distinguishes them from adult smooth muscle cells. This has led to the proposition that there are two distinct types of smooth muscle cells with different proliferative capacity. Here we systematically compare the properties of subcultured adult smooth muscle cells in culture and clonal lines of cobblestone smooth muscle cells from both neonatal rats and injured vessels. The cobblestone smooth muscle cells have a significantly smaller average cell volume, estimated using two different flow cytometry measurements. However, the two types of smooth muscle cells have indistinguishable protein expression patterns when thelevels of more than 20 different proteins (including cytoskeletal proteins, matrix proteins, cytokines, cytokine receptors, adhesion molecules and enzymes) are measured by quantitative immunofluorescence. Furthermore, in contrast to previous observations, we demonstrate that both types of smooth muscle cells secrete a powerful mitogenic activity. The higher cell density achieved by the cobblestone smooth muscle cells in culture was responsible for the earlier reports that this mitogenic activity was secreted only by cobblestone smooth muscle cells. We conclude that many of the differences seen between cobblestone smooth muscle cells and adult smooth muscle cells in vitro (proliferation rate, morphology, protein expression pattern, secretion of mitogenic activity) could be attributable to a stable difference in the median cell volume of the cultures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:08:30