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Titolo:
beta-Cyclodextrin inhibits the sweet taste suppressing activity of gurmarin by the formation of an inclusion complex with aromatic residues in gurmarin
Autore:
Imoto, T; Sasamoto, K; Ninomiya, Y;
Indirizzi:
Tottori Univ, Fac Med, Dept Physiol, Yonago, Tottori 6830826, Japan Tottori Univ Yonago Tottori Japan 6830826 Yonago, Tottori 6830826, Japan Kyushu Univ, Grad Sch Dent Sci, Sect Oral Neurosci, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ci, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
fascicolo: 10, volume: 79, anno: 2001,
pagine: 836 - 840
SICI:
0008-4212(200110)79:10<836:BITSTS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GYMNEMA-SYLVESTRE; PEPTIDE GURMARIN; RESPONSES; LEAVES;
Keywords:
sweet taste; gurmarin; Gymnema sylvestre; cyclodextrin; inclusion complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Imoto, T Tottori Univ, Fac Med, Dept Physiol, 86 Nishimachi, Yonago, Tottori 6830826, Japan Tottori Univ 86 Nishimachi Yonago Tottori Japan 6830826 26, Japan
Citazione:
T. Imoto et al., "beta-Cyclodextrin inhibits the sweet taste suppressing activity of gurmarin by the formation of an inclusion complex with aromatic residues in gurmarin", CAN J PHYSL, 79(10), 2001, pp. 836-840

Abstract

Our recent study in mice revealed that the inhibitory activity of gurmarinon the sweet taste responses was reduced significantly by the presence of beta -cyclodextrin (beta -CD). To investigate the mechanism involved in theaction of beta -CD, physicochemical experiments were performed on the interaction of CDs with gurmarin examining the effect of CDs on the UV absorption spectrum of gurmarin and on the elution behavior in gel filtration (or exclusion) chromatography. Among the three kinds of cyclodextrins tested, beta -CD induced significant changes in the UV absorption spectrum of gurmarin that were characteristic of those found in the inclusion complex formation of tyrosine and tryptophan with beta -CD. The abnormal retention behaviorof gurmarin in gel filtration resulting from hydrophobic interaction with the gel matrix reverted to normal in the presence of beta -CD in the elution buffer. These results suggest that the unique domain of gurmarin, in which five aromatic amino acid residues are all directed outwardly and form a hydrophobic cluster, is a possible site of interaction with the gurmarin-sensitive sweet taste receptor molecules in rodents.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:14:43