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Titolo:
Detection of tryptase in cytoplasmic granules of basophils in patients with chronic myeloid leukemia and other myeloid neoplasms
Autore:
Samorapoompichit, P; Kiener, HP; Schernthaner, GH; Jordan, JH; Agis, H; Wimazal, F; Baghestanian, M; Rezaie-Majd, A; Sperr, WR; Lechner, K; Valent, P;
Indirizzi:
Univ Vienna, Inst Histol & Embryol, Dept Internal Med 1, Div Hematol & Hemostaseol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 & Hemostaseol, A-1090 Vienna, Austria Univ Vienna, Dept Internal Med 2, Div Angiol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 2, Div Angiol, A-1090 Vienna, Austria Univ Vienna, Dept Internal Med 3, Div Rheumatol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Div Rheumatol, A-1090 Vienna, Austria
Titolo Testata:
BLOOD
fascicolo: 8, volume: 98, anno: 2001,
pagine: 2580 - 2583
SICI:
0006-4971(20011015)98:8<2580:DOTICG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MAST-CELLS; COMPLEMENTARY-DNA; MEDIATOR RELEASE; BONE-MARROW; DIFFERENTIATION; BIOLOGY; CLONING; TISSUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Valent, P Univ Vienna, Inst Histol & Embryol, Dept Internal Med 1, Div Hematol & Hemostaseol, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 ustria
Citazione:
P. Samorapoompichit et al., "Detection of tryptase in cytoplasmic granules of basophils in patients with chronic myeloid leukemia and other myeloid neoplasms", BLOOD, 98(8), 2001, pp. 2580-2583

Abstract

Tryptases are serine proteases primarily expressed in mast cells. Normal blood basophils express only trace amounts of the enzyme. However, recent immunohistochemical studies have raised the possibility that neoplastic basophils express significant amounts of tryptase. In this study, tryptase expression was analyzed in normal and neoplastic basophils by immunoelectron microscopy using antitryptase monoclonal antibody G3. Basophils were obtained from patients with chronic myeloid leukemia (CIVIL), idiopathic myelofibrosis (IMF), and myelodysplastic syndrome (MDS), and from healthy donors. Tryptase-immunoreactive material was detected in cytoplasmic granules of basophils in CIVIL, IMF, and MDS. By contrast, normal basophils did not contain significant amounts of tryptase by immunoelectron microscopy. As assessed byreverse transcription-polymerase chain reaction, neoplastic basophils contained messenger RNA (mRNA) for alpha -tryptase, but no beta -tryptase mRNA. In summary, these data provide evidence that neoplastic basophils in CIVIL, IMF, and MDS can express detectable amounts of tryptase. Therefore, tryptase should not be regarded as specific for mast cells when neoplastic myeloid cells are analyzed. (Blood 2001;98:2580-2583) (C) 2001 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 23:17:48