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Titolo:
Protein modifications during antiviral heat bioprocessing and subsequent storage
Autore:
Smales, CM; Pepper, DS; James, DC;
Indirizzi:
Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England Univ Kent Canterbury Kent England CT2 7NJ nterbury CT2 7NJ, Kent, England Scottish Natl Blood Transfus Serv, Edinburgh EH1 1EY, Midlothian, ScotlandScottish Natl Blood Transfus Serv Edinburgh Midlothian Scotland EH1 1EY d
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 17, anno: 2001,
pagine: 974 - 978
SICI:
8756-7938(200109/10)17:5<974:PMDAHB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
END-PRODUCTS; FACTOR-VIII; HEMOPHILIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Smales, CM Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England Univ Kent Canterbury Kent England CT2 7NJ 2 7NJ, Kent, England
Citazione:
C.M. Smales et al., "Protein modifications during antiviral heat bioprocessing and subsequent storage", BIOTECH PR, 17(5), 2001, pp. 974-978

Abstract

Antiviral heat treatment is routinely used in the bioprocessing of therapeutic proteins as a means of reducing viral load. However, in protein formulations containing sucrose this form of bioprocessing can lead to protein modifications. Using a model protein, hen egg white lysozyme, we investigatedthe effects of antiviral heat treatments in the presence of sucrose on protein integrity during subsequent long-term protein storage. Although heat treatment alone resulted in protein modification, subsequent medium- to long-term storage of both lyophilized and liquid samples at room temperature orabove led to further protein modifications. The majority of these modifications were due to the formation of glycation and advanced glycation end products via the reaction of reducing sugars and their autoxidation products (derived from hydrolyzed sucrose) with function groups on the protein surface. These findings have implications for the improvement of therapeutic protein bioprocessing to ensure protein product quality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 20:01:42