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Titolo:
Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope
Autore:
Wangsa-Wirawan, ND; Ikai, A; ONeill, BK; Middelberg, APJ;
Indirizzi:
Univ Adelaide, Dept Chem Engn, Cooperat Res Ctr Tissue Growth & Repair, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia 5005 ir, Adelaide, SA 5005, Australia Tokyo Inst Technol, Fac Biosci & Biotechnol, Biodynam Lab, Midori Ku, Yokohama, Kanagawa 226, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 226 ohama, Kanagawa 226, Japan Univ Cambridge, Dept Chem Engn, Cambridge CB2 3RA, England Univ CambridgeCambridge England CB2 3RA ngn, Cambridge CB2 3RA, England
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 17, anno: 2001,
pagine: 963 - 969
SICI:
8756-7938(200109/10)17:5<963:MTIFBP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
INORGANIC SALT-SOLUTIONS; SURFACE INTERACTIONS; AQUEOUS-ELECTROLYTE; COLLOIDAL FORCES; ADHESION; PARTICLE; SILICA; WATER; REVERSAL; FRICTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: O'Neill, BK Univ Adelaide, Dept Chem Engn, Cooperat Res Ctr Tissue Growth & Repair, Adelaide, SA 5005, Australia Univ Adelaide Adelaide SA Australia5005 , SA 5005, Australia
Citazione:
N.D. Wangsa-Wirawan et al., "Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope", BIOTECH PR, 17(5), 2001, pp. 963-969

Abstract

Interaction forces between protein inclusion bodies and an air bubble havebeen quantified using an atomic force microscope (AFM). The inclusion bodies were attached to the AFM tip by covalent bonds. Interaction forces measured in various buffer concentrations varied from 9.7 nN to 25.3 nN (+/- 4-11%) depending on pH. Hydrophobic forces provide a stronger contribution to overall interaction force than electrostatic double layer forces. It also appears that the ionic strength affects the interaction force in a complex way that cannot be directly predicted by DLVO theory. The effects of pH are significantly stronger for the inclusion body compared to the air bubble. This study provides fundamental information that will subsequently facilitate the rational design of flotation recovery system for inclusion bodies. Ithas also demonstrated the potential of AFM to facilitate the design of such processes from a practical viewpoint.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 12:28:29