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Titolo:
Comparison of protein fouling on heat-treated poly(vinyl alcohol), poly(ether sulfone) and regenerated cellulose membranes using diffuse reflectance infrared Fourier transform spectroscopy
Autore:
Amanda, A; Mallapragada, SK;
Indirizzi:
Iowa State Univ, Dept Chem Engn, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 niv, Dept Chem Engn, Ames, IA 50011 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 17, anno: 2001,
pagine: 917 - 923
SICI:
8756-7938(200109/10)17:5<917:COPFOH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERPENETRATING NETWORKS; POLY(ACRYLIC ACID); ADSORPTION; SURFACES; HYDROGEL; BIOCOMPATIBILITY; ULTRAFILTRATION; BIOSEPARATIONS; IMMOBILIZATION; PERMEATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Mallapragada, SK 1035 Sweeney Hall, Ames, IA 50011 USA 1035 Sweeney Hall Ames IA USA 50011 , Ames, IA 50011 USA
Citazione:
A. Amanda e S.K. Mallapragada, "Comparison of protein fouling on heat-treated poly(vinyl alcohol), poly(ether sulfone) and regenerated cellulose membranes using diffuse reflectance infrared Fourier transform spectroscopy", BIOTECH PR, 17(5), 2001, pp. 917-923

Abstract

Poly(vinyl alcohol) (PVA) membranes for ultrafiltration were fabricated byheat-treatment to separate macromolecules from microsolutes. PVA is a hydrophilic polymer with good mechanical properties because of its semicrystalline structure. The membranes were heat-treated at 100 degreesC for 1 h to increase their crystallinity and thereby their mechanical strength. The mechanical strength of the membranes was evaluated using a dynamic mechanical analyzer by measuring their compressive and tensile moduli. Membrane selectivities and protein fouling of heat-treated PVA membranes were compared to the commercial poly(ether sulfone) (PeS) and regenerated cellulose membranes. Myoglobin from horse skeletal muscle was used as a model protein, and L-tryptophan was used as a model microsolute. Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) was used to identify protein fouling on the surfaces of these membranes under flow and nonflow conditions. It wasfound from the selectivity and DRIFTS studies that PVA membranes were moreresistant to fouling than regenerated cellulose and PeS membranes.

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Documento generato il 02/04/20 alle ore 18:56:39