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Titolo:
New miniaturized hollow-fiber bioreactor for in vivo like cell culture, cell expansion, and production of cell-derived products
Autore:
Gloeckner, H; Lemke, HD;
Indirizzi:
Acordis Res, Obernburg, Germany Acordis Res Obernburg GermanyAcordis Res, Obernburg, Germany
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 17, anno: 2001,
pagine: 828 - 831
SICI:
8756-7938(200109/10)17:5<828:NMHBFI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIC-CELLS; IN-VITRO; DENSITY; GROWTH; PROLIFERATION; LYMPHOCYTES; DIFFERENTIATION; LINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Lemke, HD Acordis Res, Obernburg, Germany Acordis Res Obernburg GermanyAcordis Res, Obernburg, Germany
Citazione:
H. Gloeckner e H.D. Lemke, "New miniaturized hollow-fiber bioreactor for in vivo like cell culture, cell expansion, and production of cell-derived products", BIOTECH PR, 17(5), 2001, pp. 828-831

Abstract

We have developed a miniaturized hollow-fiber bioreactor system for mammalian cell culture with a volume of 1 mL. Cell and medium compartments of thebioreactor are separated by a semipermeable membrane, and oxygenation of the cell compartment is accomplished using an oxygenation membrane. As a result of the geometry of the transparent housing, cells can be observed by microscopy during culture. The leukemic cell lines CCRF-CEM, HL-60, and REH were cultivated up to densities of 3.5 x 10(7)/ mL without medium change or manipulation of the cells. As shown using CCRF-CEM cells, growth in the bioreactor was strongly influenced and could be controlled by the medium flow rate. As a consequence, consumption of glucose and generation of lactate varied with flow rate. Depending on the molecular size cutoff of the membranes used, added growth factors such as GM-CSF, as well as factors secreted from the cells, are retained in the cell compartment for up to 1 week. This new miniaturized hollow-fiber bioreactor offers advantages in tissue engineering by continuous nutrient supply for cells in high density, retention of added or autocrine produced factors, and undisturbed long-term culture in aclosed system.

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Documento generato il 09/04/20 alle ore 19:41:27