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Titolo:
'Troy-bodies': antibodies as vector proteins for T cell epitopes
Autore:
Lunde, E; Rasmussen, IB; Eidem, JK; Gregers, TF; Western, KH; Bogen, B; Sandlie, I;
Indirizzi:
Univ Oslo, Dept Biol, Div Mol Cell Biol, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316 iol, Div Mol Cell Biol, N-0316 Oslo, Norway Natl Hosp Norway, Inst Immunol, N-0027 Oslo, Norway Natl Hosp Norway Oslo Norway N-0027 y, Inst Immunol, N-0027 Oslo, Norway
Titolo Testata:
BIOMOLECULAR ENGINEERING
fascicolo: 3, volume: 18, anno: 2001,
pagine: 109 - 116
SICI:
1389-0344(20011015)18:3<109:'AAVPF>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN-PRESENTING CELLS; II-RESTRICTED PRESENTATION; MURINE IMMUNE-SYSTEM; B-CELLS; RECOMBINANT ANTIBODIES; TARGETING AUTOANTIGEN; POLYCLONAL ACTIVATION; LYMPHOCYTES-T; IN-VIVO; PEPTIDE;
Keywords:
antigen processing; MHC; antibodies; vaccination; molecular biology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Sandlie, I Univ Oslo, Dept Biol, Div Mol Cell Biol, POB 1050 Blindern, N-0316 Oslo, Norway Univ Oslo POB 1050 Blindern Oslo Norway N-0316 16 Oslo, Norway
Citazione:
E. Lunde et al., "'Troy-bodies': antibodies as vector proteins for T cell epitopes", BIOMOL ENG, 18(3), 2001, pp. 109-116

Abstract

A major objective in vaccine development is the design of reagents that give a strong, specific T cell response. Targeting of antigens to antigen presenting cells (APC) results in enhanced antigen presentation and T cell activation. In this paper, we describe a novel targeting reagent denoted 'Troy-bodies', namely recombinant antibodies with APC-specificity and with T cell epitopes integrated in their C regions. We have made such antibodies withV regions specific for either IgD or MHC class II, and five different T cell epitopes have been tested. All epitopes could be introduced into loops of C domains without disrupting immunoglobulin (Ig) folding. Four have been tested in T cell activation studies, and all could be released and presented by A-PC. Furthermore, whether IgD- or MHC-specific, the molecules tested enhanced T cell stimulation compared to non-specific control antibodies in vitro as well as in vivo. Using this technology, specific reagents can be designed that target selected antigenic peptides to an APC of choice. Troy-bodies may therefore be useful for manipulation of immune responses, and in particular for vaccination purposes. (C) 2001 Published by Elsevier ScienceB.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:46:06