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Titolo:
Zinc induces apoptosis that can be suppressed by lanthanum in C6 rat glioma cells
Autore:
Haase, H; Watjem, W; Beyersmann, D;
Indirizzi:
Univ Bremen, Fachbereich Biol Chem, D-28334 Bremen, Germany Univ Bremen Bremen Germany D-28334 ch Biol Chem, D-28334 Bremen, Germany
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 8, volume: 382, anno: 2001,
pagine: 1227 - 1234
SICI:
1431-6730(200108)382:8<1227:ZIATCB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL NEURONAL DEATH; CEREBRAL-ISCHEMIA; DNA FRAGMENTATION; DIFFERENTIATION; INHIBITION; BRAIN; ACTIVATION; INDUCTION; NECROSIS; CADMIUM;
Keywords:
apoptosis; DNA fragmentation; lanthanum; zinc;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Beyersmann, D Univ Bremen, Fachbereich Biol Chem, D-28334 Bremen, Germany Univ Bremen Bremen Germany D-28334 D-28334 Bremen, Germany
Citazione:
H. Haase et al., "Zinc induces apoptosis that can be suppressed by lanthanum in C6 rat glioma cells", BIOL CHEM, 382(8), 2001, pp. 1227-1234

Abstract

Zinc ions have both essential and toxic effects on mammalian cells. Here we report the ability of zinc to act as an inducer of apoptosis in C6 rat glioma cells. Incubation with 150 to 300 pm ZnCl2 caused cell death that was characterized as apoptotic by internucleosomal DNA fragmentation, formationof apoptotic bodies, nuclear fragmentation and breakdown of the mitochondrial membrane potential. On the other hand, zinc deprivation by the membranepermeable chelator TPEN [N,N,N ' ,N ',-tetrakis (2-pyridyl-methyl)ethylenediamine] also induced programmed death in this cell line, indicating the existence of intracellular zinc levels below and above which apoptosis is induced. Zinc-induced apoptosis in C6 cells was independent of major signalingpathways (protein kinase C, mitogen activated protein kinase and guanylatecyclase) and protein synthesis, but was increased by facilitating zinc uptake with the ionophore pyrithione. Lanthanum(III) chloride was also able toincrease the net zinc uptake, but nevertheless apoptotic features and zinctoxicity were reduced. Remarkably, lanthanum suppressed the zinc-induced breakdown of the mitochondrial membrane potential. We conclude that in C6 cells lanthanum acts in two different ways, as a promoter of net zinc uptake and as a suppressor of zinc-induced apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 12:04:51