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Titolo:
Antiproliferative activity of an aqueous mistletoe extract in human tumor cell lines and xenografts in vitro
Autore:
Burger, AM; Mengs, U; Schuler, JB; Fiebig, HH;
Indirizzi:
Madaus AG, D-51109 Cologne, Germany Madaus AG Cologne Germany D-51109Madaus AG, D-51109 Cologne, Germany Univ Freiburg, Tumor Biol Ctr, Freiburg, Germany Univ Freiburg Freiburg Germany iburg, Tumor Biol Ctr, Freiburg, Germany
Titolo Testata:
ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH
fascicolo: 9, volume: 51, anno: 2001,
pagine: 748 - 757
SICI:
0004-4172(2001)51:9<748:AAOAAM>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LECTIN-I; HUMAN MONOCYTES; A-CHAIN; CYTOTOXICITY; LYMPHOCYTES; APOPTOSIS; ASSAY; STIMULATION; INDUCTION; CYTOKINES;
Keywords:
CAS 23214-92-8; doxorubicin; lektinol; mistletoe extract, antiproliferative activity in vitro, human tumor cell lines, xenografts; mistletoe lectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Mengs, U Madaus AG, Ostmerheimer Str 198, D-51109 Cologne, Germany Madaus AG Ostmerheimer Str 198 Cologne Germany D-51109 , Germany
Citazione:
A.M. Burger et al., "Antiproliferative activity of an aqueous mistletoe extract in human tumor cell lines and xenografts in vitro", ARZNEI-FOR, 51(9), 2001, pp. 748-757

Abstract

The in vitro antiproliferative activity of an aqueous mistletoe extract (AME) with a defined content of bioactive mistletoe lectin (ML) was tested in25 human tumor cell lines, including 20 solid and 5 hematological malignancies and 47 human tumor xenografts. The antiproliferative activity of AME was compared to that of the standard cytotoxic: agent doxorubicin (CAS 23214-92-8, adriamycin, ADR) using the sulforhodamin B, propidium iodide and soft agar colony forming assays, respectively. AME was highly cytotoxic in solid human tumors with mean IC70 values in the range of 0.17 - 1 ng ML/ml (2.8-17 pmol bioactive ML). On a molar basis, AME was 3 to 4 logs more potent than ADR and showed differential cytotoxicity towards tumors of the breast,small cell and non-small cell lung, prostate and renal cell cancers. AME was also highly active in hematological malignancies with steep dose response curves resulting in mean IC70 values of 0.12 ng ML/ml (2 pmol). The acutelymphoblastic leukemia cell line HL-60 was the most sensitive, the histiocytic lymphoma cell line U937 the most resistant hematological malignancy. It Is important to stress that AME did not induce a biologically relevant increase of cell proliferation in any of the tumor cell lines tested. Our data suggest that AME has in vitro antitumor profiles similar to thoseof classical anticancer agents. Clear dose-response relationships were found in all of the performed experiments and Interesting differential cytotoxicity patterns were observed. Experiments with sensitive tumor types identified in these in vitro studies are currently ongoing in order to demonstrate the anticancer activity of AME in different animal tumor models.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:57:42