Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A morphologic, biochemical, and biomechanical assessment of short-term effects of osteochondral autograft plug transfer in an animal model
Autore:
Lane, JG; Tontz, WL; Ball, ST; Massie, JB; Chen, AC; Bae, WC; Amiel, ME; Sah, RL; Amiel, D;
Indirizzi:
Univ Calif San Diego, Dept Orthopaed, Connect Tissue Biochem Labs, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 m Labs, La Jolla, CA 92093 USA Univ Calif San Diego, Dept Bioengn, Cartilage Tissue Engn Labs, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 n Labs, La Jolla, CA 92093 USA
Titolo Testata:
ARTHROSCOPY
fascicolo: 8, volume: 17, anno: 2001,
pagine: 856 - 863
SICI:
0749-8063(200110)17:8<856:AMBABA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE; FOLLOW-UP; KNEE; ARTHROSCOPY; LESIONS;
Keywords:
cartilage repair; osteochondral defect; osteochondral autograft plug transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Amiel, D Univ Calif San Diego, Dept Orthopaed, Connect Tissue Biochem Labs, 9500 Gilman Dr, La Jolla, CA 92093 USA Univ Calif San Diego 9500 Gilman Dr La Jolla CA USA 92093 093 USA
Citazione:
J.G. Lane et al., "A morphologic, biochemical, and biomechanical assessment of short-term effects of osteochondral autograft plug transfer in an animal model", ARTHROSCOPY, 17(8), 2001, pp. 856-863

Abstract

Purpose: The objective of this study was to assess the short-term. changesthat occur after an osteochondral autograft plug transfer from the femoraltrochlea to the media femoral condyle in a goat model. Type of Study: Articular cartilage repair animal study. Methods: Six adult male goats were used in this study. Two 4.5-mm osteochondral plugs were transferred from the superolateral femoral trochlea to 2 recipient sites in the central portion of the medial femoral condyle for a survival period of 12 weeks. Postmortem,the global effects of the procedure were assessed by gross morphologic inspection and by analyzing the synovial DNA for inflammatory response. The recipient sites were also evaluated histologically and biomechanically. Metabolic activity was determined by (SO4)-S-35 uptake, and viability assessed using a live/dead stain and by confocal laser microscopy. Results: There wasno evidence of significant gross morphologic or histologic changes in the operative knee as a result of the osteochondral donor or recipient sites. The patella, tibial plateau, and medial meniscus did not show any increased degenerative changes as a result of articulating against the donor or recipient sites of the osteochondral autografts. Analysis of synovial DNA revealed no inflammatory response, Biomechanically, 6- to 7-fold greater stiffness was noted in the cartilage of the transferred plugs compared with the control medial femoral condyle. Furthermore, on histologic examination, the healing subchondral bone interface at the recipient site had increased density. Glycosaminoglycan synthesis as determined by (SO4)-S-35 uptake was upregulated in the transplanted cartilage plug relative to the contralateral control, showing a repair response at the site of implantation. And finally, confocal microscopy showed 95% viability of the transferred plugs in the medial femoral condyle region. Conclusions: Our findings demonstrate the ability to successfully transfer an osteochondral autograft plug with maintenance of chondrocyte cellular viability. The transferred cartilage is stiffer than the control media] femoral condyle cartilage, and there is concern regarding the increased trabecular mass in the healing subchondral plate, but these do not result in increased degenerative chancres of the opposing articular surfaces in the short term.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:43:14