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Titolo:
Assessment of chitin decomposer diversity within an upland grassland
Autore:
Krsek, M; Wellington, EMH;
Indirizzi:
Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England
Titolo Testata:
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
fascicolo: 3-4, volume: 79, anno: 2001,
pagine: 261 - 267
SICI:
0003-6072(2001)79:3-4<261:AOCDDW>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENES; SOIL; FUNGI;
Keywords:
actinomycetes; chitinase; DGGE; grassland ecology; litter bags; soil microcosms;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Krsek, M Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL dlands, England
Citazione:
M. Krsek e E.M.H. Wellington, "Assessment of chitin decomposer diversity within an upland grassland", ANTON LEEUW, 79(3-4), 2001, pp. 261-267

Abstract

The breakdown of chitin within an acidic upland grassland was studied. Theaim was to provide a molecular characterisation of microorganisms involvedin chitin degradation in the soil using soil microcosms and buried litter bags containing chitin. The investigation involved an examination of the effects of liming on the microbial communities within the soil and their chitinolytic activity. Microcosm experiments were designed to study the influence of lime and chitin enrichment on the grassland soil bacterial community ex situ under controlled environmental conditions. Bacterial and actinomycete counts were determined and total community DNA was extracted from the microcosms and from chitin bags buried at the experimental site. PCR based onspecific 16S rRNA target sequences provided products for DGGE analysis to determine the structure of bacterial and actinomycete communities. Chitinase activity was assessed spectrophotometrically using chitin labelled with remazol brilliant violet. Both liming and chitin amendment increased bacterial and actinomycete viable counts and the chitinase activity. DGGE band patterns confirmed changes in bacterial populations under the influence of both treatments. PCR products amplified from DNA isolated from chitin bags were cloned and sequenced. Only a few matched known species but a prominent coloniser of chitin proved to be Stenotrophomonas maltophilia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:04:13