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Titolo:
Direct and indirect bacterial killing functions of neutrophil defensins inlung explants
Autore:
Porro, GA; Lee, JH; De Azavedo, J; Crandall, I; Whitehead, T; Tullis, E; Ganz, T; Liu, MY; Slutsky, AS; Zhang, HB;
Indirizzi:
St Michaels Hosp, Dept Anaesthesia, Div Resp, Toronto, ON M5B 1W8, Canada St Michaels Hosp Toronto ON Canada M5B 1W8 p, Toronto, ON M5B 1W8, Canada St Michaels Hosp, Dept Crit Care Med, Div Resp, Toronto, ON M5B 1W8, Canada St Michaels Hosp Toronto ON Canada M5B 1W8 p, Toronto, ON M5B 1W8, Canada Mt Sinai Hosp, Dept Microbiol, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp Toronto ON Canada M5G 1X5 biol, Toronto, ON M5G 1X5, Canada Univ Toronto, Toronto Gen Hosp, Dept Lab Med & Pathobiol, Toronto, ON M5G 2C4, Canada Univ Toronto Toronto ON Canada M5G 2C4 obiol, Toronto, ON M5G 2C4, Canada Univ Toronto, Toronto Gen Hosp, Div Thorac Surg, Toronto, ON M5G 2C4, Canada Univ Toronto Toronto ON Canada M5G 2C4 Surg, Toronto, ON M5G 2C4, Canada Univ Calif Los Angeles, Los Angeles Med Ctr, Dept Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: L1240 - L1247
SICI:
1040-0605(200111)281:5<L1240:DAIBKF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-PEROXIDE; NADPH OXIDASE; PEPTIDE DEFENSINS; ENDOTHELIAL-CELLS; PROTEINS; DIPHENYLENEIODONIUM; PURIFICATION; MENINGITIS; ACTIVATION; PLASMA;
Keywords:
reactive oxygen species; host defense; antioxidant; NADPH oxidase; lung injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, HB Univ Toronto, Dept Anaesthesia, Med Sci Bldg,Rm 7334,1 Kings Coll Circle, Toronto, ON M5S 1A8, Canada Univ Toronto Med Sci Bldg,Rm 7334,1 Kings Coll Circle Toronto ON Canada M5S 1A8
Citazione:
G.A. Porro et al., "Direct and indirect bacterial killing functions of neutrophil defensins inlung explants", AM J P-LUNG, 281(5), 2001, pp. L1240-L1247

Abstract

Studies of the antimicrobial activity of neutrophil defensins have mostly been carried out in microbiological media, and their effects on the host defense in physiological conditions are unclear. We examined 1) the antibacterial activity of defensins in physiological media with and without lung tissue present, 2) the effect of defensins on hydrogen peroxide (H2O2) production by lung tissue that had been exposed to bacteria, and 3) the effect of diphenyleneiodonium (DPI), an inhibitor of reactive oxygen species formation, on the antibacterial activity of defensins in the presence of lung tissue. Defensins were incubated with Escherichia coli or Pseudomonas aeruginosain the absence or presence of primary cultured mouse lung explants. Defensins reduced bacterial counts by similar to 65-fold and similar to 25-fold, respectively, at 48 h; bacterial counts were further decreased by similar to 600-fold and similar to 12,000-fold, respectively, in the presence of lung tissue. Defensins induced H2O2 production by lung tissue, and the rate ofkilling of E. coli by defensins was reduced by similar to2,500- fold in the presence of 10 mu MDPI. We conclude that defensins exert a significant antimicrobial effect under physiological conditions and that this effect is enhanced in the presence of lung tissue by a mechanism that involves the production of reactive oxygen species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:46:12